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首页> 外文期刊>Infection and immunity >Effects of temperature stress on expression of fimbriae and superoxide dismutase by Porphyromonas gingivalis.
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Effects of temperature stress on expression of fimbriae and superoxide dismutase by Porphyromonas gingivalis.

机译：温度胁迫对牙龈卟啉单胞菌菌毛和超氧化物歧化酶表达的影响。

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摘要

We examined the biosynthesis of fimbriae and superoxide dismutase (SOD) produced by the periodontopathic bacterium Porphyromonas gingivalis in response to elevated temperature. P. gingivalis 2561, grown at 37 degrees C to mid-logarithmic phase, was subsequently incubated at 39, 41, and 43 degrees C, respectively, to stationary phase. There was no difference in the growth of cells at 37 and 39 degrees C. However, at 39 degrees C there was a 54% reduction in the amount of fimbrillin (fimbriae) as well as decreased expression of mRNA for fimA. On the other hand, under the same conditions, a more than twofold increase in the amount of SOD activity, as well as in the levels of SOD mRNA, was observed. Moreover, cells cultured for 20 h at 39 degrees C showed an 86% decrease of fimbrillin protein and a threefold increase in SOD activity. These observations suggest that P. gingivalis may undergo alterations in its virulence and susceptibility to host immune responses as a result of the elevated temperatures found in inflamed periodontal pockets.
机译：我们检查了牙周病菌牙龈卟啉单胞菌响应温度升高而产生的菌毛和超氧化物歧化酶（SOD）的生物合成。在37℃下生长至对数中期的牙龈卟啉单胞菌2561随后分别在39、41和43℃下孵育至固定相。在37和39摄氏度时，细胞的生长没有差异。但是，在39摄氏度时，纤丝蛋白（菌丝）的量减少了54％，而fimA的mRNA表达下降。另一方面，在相同条件下，观察到SOD活性的量以及SOD mRNA的水平增加了两倍以上。此外，在39摄氏度下培养20小时的细胞显示出纤细蛋白减少了86％，SOD活性增加了三倍。这些观察结果表明，由于发炎的牙周袋中温度升高，牙龈卟啉单胞菌的毒力和对宿主免疫反应的敏感性可能发生变化。

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