• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Immunological variants of the aerobactin-cloacin DF13 outer membrane protein receptor IutA among enteric bacteria.
【24h】

Immunological variants of the aerobactin-cloacin DF13 outer membrane protein receptor IutA among enteric bacteria.

机译：肠细菌中的航空细菌素-松弛素DF13外膜蛋白受体IutA的免疫学变异。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mouse monoclonal antibodies (MAbs) were generated against a 76-kDa IutA receptor of pathogenic avian Escherichia coli 15972. Six of the eight IutA-specific MAbs isolated (AB1 to AB6) were shown to be directed toward membrane-exposed conformational epitopes, although they did not interfere with the uptake of ferric aerobactin and cloacin DF13 as assessed by competition experiments with purified ligands. The two remaining IutA MAbs (AB9 and AB10) recognized linear epitopes buried in the IutA molecule. The panel of IutA MAbs was used to characterize IutA variants occurring in strains of E. coli, Klebsiella pneumoniae, Enterobacter spp., and Shigella spp., resulting in the identification of four immunological groups of IutAs. MAb AB9 defined an epitope conserved in all IutA variants. In addition, the panel of IutA MAbs served to identify the presence of IutA in wild-type bacteria grown in the presence of diphenylamine to reduce the expression of O-specific polysaccharide.
机译：产生了针对病原性禽类大肠杆菌15972的76 kDa IutA受体的小鼠单克隆抗体（MAb）。分离出的8种IutA特异性MAb中有6种（AB1至AB6）针对膜暴露的构象表位，尽管它们通过与纯化的配体的竞争实验评估，其不干扰铁氧合细菌素和cloacin DF13的摄取。剩下的两个IutA MAb（AB9和AB10）识别埋在IutA分子中的线性表位。 IutA MAb小组用于鉴定在大肠杆菌，肺炎克雷伯菌，肠杆菌属和志贺氏菌属菌株中出现的IutA变体，从而鉴定了IutAs的四个免疫学组。 MAb AB9定义了在所有IutA变体中均保守的表位。另外，IutA MAb小组用于鉴定在二苯胺存在下生长的野生型细菌中IutA的存在，以减少O特异性多糖的表达。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号