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首页> 外文期刊>Infection and immunity >Passive immunization with monoclonal antibodies against Porphyromonas gingivalis in patients with periodontitis.
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Passive immunization with monoclonal antibodies against Porphyromonas gingivalis in patients with periodontitis.

机译：牙周炎患者中针对牙龈卟啉单胞菌的单克隆抗体的被动免疫。

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Selective inhibition of recolonization of Porphyromonas gingivalis was investigated by topical application of monoclonal antibody (MAb). To select a MAb to P. gingivalis with the potential for recognizing most strains of P. gingivalis, we examined seven MAbs, one of which (MAb 61BG 1.3) recognized all 22 laboratory strains and serotypes of P. gingivalis tested as well as 105 human clinical isolates. A comparative study of the number of P. gingivalis bacteria identified by conventional culture and immunofluorescence with MAb 61BG 1.3 showed a very significant correlation between the two methods (Spearman r = 0.85, P < 0.001). Fourteen patients with periodontitis, who harbored P. gingivalis in their subgingival plaque, were treated by root planing and with metronidazole to suppress any detectable P. gingivalis. In this double-blind study, the patients were then divided randomly into two groups; one was treated with MAb to P. gingivalis, and the other was treated with saline. Each patient had four subgingival applications of 3 micrograms of MAb (or saline) per tooth at 1, 3, 7, and 10 days after P. gingivalis was suppressed. The number of P. gingivalis bacteria was then monitored, and significantly less recolonization of the sites with the most severe periodontitis was found in the MAb-treated patients than in the control patients (P < 0.01). This was evident at 6 and 9 months after the application of MAb, but by 12 months, P. gingivalis, was also found to recolonize these sites in two of the MAb-treated patients. The effect of MAb was specific to P. gingivalis, since the numbers of spirochetes were not significantly different between the two groups. However, no significant difference in any clinical periodontal indices between the immunized and control patients at 6 and 12 months was observed. This is the first demonstration that a putative periodontal pathogen can be selectively prevented from recolonization for up to 9 months in sites with the most severe periodontitis. This strategy could be used to establish directly in humans whether a microorganism is involved in the pathogenesis of periodontitis, by repeated application of the corresponding MAb at about 6-month intervals and by comparing the clinical indices between the MAb-treated and control patients.
机译：通过局部应用单克隆抗体（MAb）研究了牙龈卟啉单胞菌再定殖的选择性抑制。为了选择具有识别大多数牙龈卟啉单胞菌菌株潜能的单克隆抗体，我们检查了七种单克隆抗体，其中一种（MAb 61BG 1.3）识别了测试的所有22种实验室菌株和血清型的牙龈卟啉单胞菌，以及105个人临床分离株。通过常规培养和用MAb 61BG 1.3进行免疫荧光鉴定的牙龈卟啉单胞菌细菌数量的比较研究表明，两种方法之间存在非常显着的相关性（Spearman r = 0.85，P <0.001）。对十四名牙周炎患者在其龈下斑块中携带牙龈卟啉单胞菌进行了根治术并用甲硝唑治疗，以抑制任何可检测到的牙龈卟啉单胞菌。在这项双盲研究中，将患者随机分为两组。一种用MAb治疗牙龈卟啉单胞菌，另一种用盐水治疗。每位患者在抑制牙龈卟啉单胞菌后的1、3、7和10天，每牙均进行4次龈下施用，每颗牙齿3微克MAb（或盐水）。然后监测牙龈卟啉单胞菌细菌的数目，并且与对照患者相比，在用MAb治疗的患者中发现最严重的牙周炎部位的再定植明显更少（P <0.01）。这在应用单克隆抗体后6个月和9个月时很明显，但是到12个月时，还发现了牙龈卟啉单胞菌在两名接受单克隆抗体治疗的患者中重新定殖了这些部位。 MAb的作用特定于牙龈卟啉单胞菌，因为两组之间的螺旋体数目没有显着差异。然而，在6个月和12个月时，免疫组和对照组的任何临床牙周指数均无显着差异。这是第一个证明，可以在最严重的牙周炎部位选择性地将假定的牙周病原体在长达9个月的时间内防止其重新定殖。通过以大约6个月的间隔重复应用相应的单克隆抗体，并通过比较接受单克隆抗体治疗的患者与对照患者之间的临床指标，可以将这种策略直接用于确定人类是否患有牙周炎。

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