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首页> 外文期刊>Infection and immunity >Bacterial virulence versus host resistance in the urinary tracts of mice.
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Bacterial virulence versus host resistance in the urinary tracts of mice.

机译：小鼠泌尿道中细菌毒性与宿主抗性的关系。

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The relative contributions of host resistance and bacterial virulence were analyzed in a mouse model for ascending urinary tract infection. The congenic mouse strains C3H/HeJ and C3H/HeN were used in parallel. They differ in their reactivity to lipopolysaccharide (LPS) and susceptibility to experimental urinary tract infection. C3H/HeJ cells are susceptible to infection and are nonresponders to LPS (Lpsd Lpsd), whereas C3H/HeN cells respond to LPS and are resistant to infection (Lpsn Lpsn). The Escherichia coli pyelonephritis isolate GR-12, serotype O75K5, expressing adhesins specific for globoseries glycolipids (P fimbriae) and for mannosides (type-1 fimbriae), and its derivatives deficient in these factors were used, either singly or in combination, to establish experimental infections. In C3H/HeN mice, the relative persistence of E. coli was inversely proportional to its phagocytosis in vitro. Loss of the O75 and K5 antigens increased the tendency toward hydrophobic interaction, promoted phagocytosis, and reduced persistence in the kidneys. This was not the case in C3H/HeJ mice, in which O75- and K5- serotypes persisted in the same extent as did the parent strain. The total number of bacteria recovered from the kidneys of C3H/HeJ mice was about 1,000-fold higher than the number recovered from kidneys of C3H/HeN mice 24 h after infection. Previous studies have demonstrated a delayed influx of polymorphonuclear leukocytes into the urinary tracts of C3H/HeJ mice. The results are consistent with the hypothesis that phagocyte activation through LPS is a major defense mechanism against E. coli in the kidney, a property in which C3H/HeJ mice are deficient.
机译：在小鼠模型中，分析了宿主抵抗力和细菌毒力对泌尿道感染的相对贡献。平行使用同系小鼠品系C3H / HeJ和C3H / HeN。它们对脂多糖（LPS）的反应性和对实验性尿路感染的敏感性不同。 C3H / HeJ细胞易受感染，对LPS无反应（Lpsd Lpsd），而C3H / HeN细胞对LPS有反应且对感染有抵抗力（Lpsn Lpsn）。大肠杆菌肾盂肾炎分离株GR-12（血清型O75K5）表达对球蛋白系列糖脂（P菌毛）和甘露糖苷（1型菌毛）具有特异性的粘附素，并单独或组合使用缺乏这些因子的衍生物来建立实验性感染。在C3H / HeN小鼠中，大肠杆菌的相对持久性与其体外吞噬作用成反比。 O75和K5抗原的丢失增加了疏水相互作用的趋势，促进了吞噬作用，并降低了肾脏的持久性。在C3H / HeJ小鼠中情况并非如此，其中O75型和K5-型血清型持续存在的程度与亲本菌株相同。从C3H / HeJ小鼠的肾脏中回收的细菌总数比感染后24小时从C3H / HeN小鼠的肾脏中回收的细菌数高约1,000倍。先前的研究表明，多形核白细胞延迟流入C3H / HeJ小鼠的尿道。该结果与以下假设相符：通过LPS吞噬细胞激活是针对肾脏中大肠杆菌的主要防御机制，而C3H / HeJ小鼠则缺乏这种特性。

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