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Mapping and Identification of the Major Cell Wall-Associated Components of Mycobacterium leprae

机译：麻风分枝杆菌主要细胞壁相关成分的定位与鉴定

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Mycobacterium leprae, an obligate intracellular pathogen, can be derived only from host tissue and thus affords the opportunity to study in vivo-expressed products responsible for the particular pathogenesis of leprosy. Despite considerable progress in the characterization of the proteins and secondary gene products ofM. leprae, there is little information on the nature of the proteins associated with the cell envelope. M. leprae has been fractionated into its major subcellular components, cell wall, cytoplasmic membrane, and soluble cytosol. A number of biochemical markers, including diaminopimelic acid content, monosaccharide composition, mycolic acid, and glycolipid distribution, were applied to their characterization, and two-dimensional gel electrophoresis was used to map the component proteins. A total of 391 major proteins spots were resolved, and 8 proteins were identified based on their reactivity to a panel of monoclonal antibodies and/or relative pI size. Microsequencing of six protein spots present in the cell wall fraction allowed identification of new proteins, including the protein elongation factor EF-Tu and a homolog for the Mycobacterium tuberculosis MtrA response regulator. These results, together with previous studies, contribute to the progressive knowledge of the composition of the in vivo-expressed proteins of M. leprae.
机译：麻风分枝杆菌是专一的细胞内病原体，只能来源于宿主组织，因此为研究麻风病特殊发病机理的体内表达产物提供了机会。尽管在表征 M的蛋白质和二级基因产物方面取得了很大进展。麻风，关于与细胞包膜相关的蛋白质性质的信息很少。 M。麻风病已被分为主要的亚细胞成分，细胞壁，细胞质膜和可溶性细胞质。包括二氨基庚二酸含量，单糖组成，霉菌酸和糖脂分布在内的许多生化标记物均已用于其表征，并使用二维凝胶电泳法绘制了组成蛋白。总共解决了391个主要蛋白质斑点，并根据它们与一组单克隆抗体的反应性和/或相对pI大小确定了8个蛋白质。对细胞壁部分中六个蛋白质斑点的微测序可以鉴定新蛋白质，包括蛋白质延伸因子EF-Tu和结核分枝杆菌MtrA反应调节剂的同源物。这些结果与以前的研究一起有助于逐步了解 M在体内表达的蛋白质的组成。麻风病。

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