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首页> 外文期刊>Infection and immunity >Switching of Flagellar Motility in Helicobacter pyloriby Reversible Length Variation of a Short Homopolymeric Sequence Repeat in fliP, a Gene Encoding a Basal Body Protein
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Switching of Flagellar Motility in Helicobacter pyloriby Reversible Length Variation of a Short Homopolymeric Sequence Repeat in fliP, a Gene Encoding a Basal Body Protein

机译：幽门螺杆菌鞭毛运动的切换短可重复序列fliP，编码基础体蛋白的基因中的短均聚序列的长度变化。

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The genome of Helicobacter pylori contains numerous simple nucleotide repeats that have been proposed to have regulatory functions and to compensate for the conspicuous dearth of master regulatory pathways in this highly host-adapted bacterium. H. pylori strain 26695, whose genomic sequence was determined by The Institute for Genomic Research (TIGR), contains a repeat of nine cytidines in the fliP flagellar basal body gene that splits the open reading frame in two parts. In this work, we demonstrate that the 26695C9 strain with a split fliP gene as sequenced by TIGR was nonflagellated and nonmotile. In contrast, earlier isolates of strain 26695 selected by positive motility testing as well as pig-passaged derivatives of 26695 were all flagellated and highly motile. All of these motile strains had a C8 repeat and consequently a contiguous fliP reading frame. By screening approximately 50,000 colonies of 26695C9 for motility in soft agar, a motile revertant with a C8 repeat could be isolated, proving that the described switch is reversible. ThefliP genes of 20 motile clinical H. pyloriisolates from different geographic regions possessed intactfliP genes with repeats of eight cytidines or the sequence CCCCACCC in its place. Isogenic fliP mutants of a motile, C8 repeat isolate of strain 26695 were constructed by allelic exchange mutagenesis and found to be defective in flagellum biogenesis. Mutants produced only small amounts of flagellins, while the transcription of flagellin genes appeared unchanged. These results strongly suggest a unique mechanism regulating motility in H. pylori which relies on slipped-strand mispairing-mediated mutagenesis of fliP.
机译：幽门螺杆菌的基因组包含许多简单的核苷酸重复序列，这些重复序列被认为具有调控功能，可以补偿这种高度宿主适应性细菌中主要调控途径的明显缺乏。 H。 pylori 菌株26695，其基因组序列由基因组研究所（TIGR）确定，在 fliP 鞭毛基体基因中包含9个胞苷的重复序列，该基因可将开放阅读框分开。两个部分。在这项工作中，我们证明了通过TIGR测序的具有 fliP 分裂基因的266​​95 C9 菌株是无鞭毛且不运动的。相反，通过阳性运动试验选择的早期菌株26695分离株，以及经过猪的26695传代衍生物都具有鞭毛，并且具有很高的运动力。所有这些运动菌株均具有C 8 重复序列，因此具有连续的 fliP 阅读框。通过在软琼脂中筛选大约50,000个菌落的26695 C9 的运动性，可以分离出具有C 8 重复序列的能动性回复子，证明所描述的开关是可逆的。 20个活动性临床 H的 fliP 基因。来自不同地理区域的幽门螺杆菌分离物具有完整的 fliP 基因，其中有8个胞苷或CCCCACCC序列的重复。通过等位基因交换诱变构建了能动的26695株C 8 重复菌株的同基因 fliP 突变体，发现鞭毛生物发生缺陷。突变体仅产生少量鞭毛蛋白，而鞭毛蛋白基因的转录似乎没有变化。这些结果强烈暗示了调节 H运动性的独特机制。 pylori 依赖于滑链错配对介导的 fliP 诱变。

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