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首页> 外文期刊>Infection and immunity >Evidence for Acquisition of the Lipooligosaccharide Biosynthesis Locus in Campylobacter jejuni GB11, a Strain Isolated from a Patient with Guillain-Barré Syndrome, by Horizontal Exchange
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Evidence for Acquisition of the Lipooligosaccharide Biosynthesis Locus in Campylobacter jejuni GB11, a Strain Isolated from a Patient with Guillain-Barré Syndrome, by Horizontal Exchange

机译：空肠弯曲杆菌GB11中的脂寡糖生物合成基因座的获取证据，该菌株是通过水平交换从患有格林-巴利综合征的患者中分离的菌株

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Campylobacter jejuni GB11, a strain isolated from a patient with Guillain-Barré syndrome, has been shown to be genetically closely related to the completely sequenced strain C. jejuni NCTC 11168 by various molecular typing and serotyping methods. However, we observed that the lipooligosaccharide (LOS) biosynthesis genes strongly diverged between GB11 and NCTC 11168. We sequenced the LOS biosynthesis locus of GB11 and found that it was nearly identical to the class A LOS locus from the C. jejuni HS:19 Penner serotype strain (ATCC 43446). Analysis of the DNA sequencing data showed that a horizontal exchange event involving at least 14.26 kb had occurred in the LOS biosynthesis locus of GB11 between galE (Cj1131c in NCTC 11168) and gmhA (Cj1149 in NCTC 11168). Mass spectrometry of the GB11 LOS showed that GB11 expressed an LOS outer core that mimicked the carbohydrate portion of the gangliosides GM1a and GD1a, similar to C. jejuni ATCC 43446. The serum from the GB11-infected patient was shown to react with the LOS from both GB11 and ATCC 43446 but not with that from NCTC 11168. These data indicate that the antiganglioside response in the GB11-infected patient was raised against the structures synthesized by the acquired class A LOS locus.
机译：空肠弯曲杆菌 GB11是从吉兰-巴雷综合征患者中分离出来的一种菌株，在基因上与完全测序的菌株 C密切相关。空肠NCTC 11168通过各种分子分型和血清分型方法。但是，我们观察到脂蛋白寡糖（LOS）的生物合成基因在GB11和NCTC 11168之间有很大差异。我们对GB11的LOS生物合成基因座进行了测序，发现它与 C的A类LOS基因座几乎相同。空肠HS：19 Penner血清型菌株（ATCC 43446）。对DNA测序数据的分析表明，在 galE （NCTC 11168中为Cj1131c）和 gmhA 之间，GB11的LOS生物合成位点发生了至少14.26 kb的水平交换事件。 （NCTC 11168中的Cj1149）。 GB11 LOS的质谱分析表明，GB11表达的LOS外核模仿了神经节苷脂GM1a和GD1a的碳水化合物部分，类似于 C。空肠ATCC43446。显示GB11感染患者的血清与GB11和ATCC 43446的LOS反应，但与NCTC 11168的LOS不反应。这些数据表明，GB11感染患者的抗神经节苷脂反应相对于由获得的A类LOS位点合成的结构进行了研究。

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