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首页> 外文期刊>Infection and immunity >Distribution of a protein antigenically related to the major anaerobically induced gonococcal outer membrane protein among other Neisseria species.
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Distribution of a protein antigenically related to the major anaerobically induced gonococcal outer membrane protein among other Neisseria species.

机译：在其他奈瑟氏球菌属物种中，与主要厌氧诱导的淋球菌外膜蛋白抗原相关的蛋白质分布。

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The Pan 1 protein of Neisseria gonorrhoeae is a novel 54-kDa outer membrane protein expressed only when gonococci are grown in the absence of oxygen. It is a major antigen recognized by sera from patients with gonococcal infection. We raised mouse monospecific polyclonal antiserum to gel-purified Pan 1 from gonococcal strain F62. The antiserum was broadly cross-reactive among gonococcal strains; all strains tested reacted in immunoblot analysis proportionate to the amount of Pan 1 visible in silver-stained sodium dodecyl sulfate (SDS)-polyacrylamide gels. In immunoblot experiments, N. lactamica and N. cinerea reacted very strongly to the anti-Pan 1 antiserum, whereas N. sicca, N. flava, and N. mucosa did not react at all. The other commensals tested, N. subflava and N. perflava, exhibited only a minor reaction. These results correlated with the apparent amount of Pan 1 seen on SDS-polyacrylamide gels of outer membranes. SDS-polyacrylamide gel analysis of six meningococcal strains revealed no visible anaerobically induced outer membrane proteins, and the subsequent immunoblots showed only slight or no reaction to the anti-Pan 1 antibody. In the four meningococcal strains that did react slightly with the antiserum, a Pan 1-like protein was seen only in anaerobically grown cells. Thus, meningococci did not express Pan 1 at levels comparable to that found in gonococci; however, when Pan 1 was expressed in meningococcal strains, it was oxygen regulated. This is the first example of a protein found in the gonococcal outer membrane that, under identical growth conditions, is not expressed at similar levels in the meningococcus.
机译：淋病奈瑟氏球菌的Pan 1蛋白是一种新型的54 kDa外膜蛋白，仅在淋球菌在无氧条件下生长时才表达。它是淋病球菌感染患者血清中识别的主要抗原。我们将小鼠单特异性多克隆抗血清从淋球菌F62菌株中纯化至凝胶纯化的Pan 1。抗血清在淋球菌菌株之间具有广泛的交叉反应性。所有测试的菌株在免疫印迹分析中的反应均与在银染的十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶中可见的Pan 1的量成比例。在免疫印迹实验中，内酰胺猪笼草和灰质猪笼草对抗Pan 1抗血清反应非常强烈，而西卡猪笼草，黄花猪笼草和粘膜猪笼草则根本不反应。所测试的其他推荐，N。subflava和N. perflava仅表现出较小的反应。这些结果与在外膜的SDS-聚丙烯酰胺凝胶上看到的Pan 1的表观量相关。对六个脑膜炎球菌菌株的SDS-聚丙烯酰胺凝胶分析显示，没有可见的厌氧诱导的外膜蛋白，随后的免疫印迹显示，与抗Pan 1抗体的反应很小或没有反应。在确实与抗血清发生轻微反应的四种脑膜炎球菌菌株中，仅在厌氧生长的细胞中发现了Pan 1样蛋白。因此，脑膜炎球菌不表达Pan 1的水平与淋球菌相当。但是，当Pan 1在脑膜炎球菌菌株中表达时，它是受氧调节的。这是在淋球菌外膜中发现的蛋白质的第一个例子，该蛋白质在相同的生长条件下不会在脑膜炎球菌中以相似的水平表达。

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