Sequence analysis and expression in Escherichia coli of the hyaluronidase gene of Streptococcus pyogenes bacteriophage H4489A.

W L Hynes; J J Ferretti

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Sequence analysis and expression in Escherichia coli of the hyaluronidase gene of Streptococcus pyogenes bacteriophage H4489A.

机译：化脓性链球菌噬菌体H4489A的透明质酸酶基因的序列分析和在大肠杆菌中的表达。

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The hyaluronidase gene (hylP) from Streptococcus pyogenes bacteriophage H4489A was previously cloned into Escherichia coli plasmid pUC8 as a 3.1-kilobase ThaI fragment. Southern hybridization experiments confirmed the origin of this fragment in bacteriophage H4489A before determination of the nucleotide sequence of the entire fragment. Two open reading frames (ORFs) were found, the first of which specified a 39,515-molecular-weight protein identified as the bacteriophage hyaluronidase. The second ORF encoded a 65,159-molecular-weight protein of unknown function. Putative transcription and translation control sequences for each ORF were identified by using a plasmid containing a promoterless chloramphenicol acetyltransferase gene. Controlled exclusive expression of the hylP gene via the T7 polymerase-promoter system in E. coli resulted in a 40,000-dalton protein, a result consistent with the coding capacity of the hylP gene.

机译：来自化脓性链球菌噬菌体H4489A的透明质酸酶基因（hylP）先前作为3.1碱基碱基的ThaI片段被克隆到大肠杆菌质粒pUC8中。 Southern杂交实验在确定整个片段的核苷酸序列之前在噬菌体H4489A中证实了该片段的起源。发现了两个开放阅读框（ORF），第一个开放阅读框指定了一种分子量为39,515的蛋白质，被鉴定为噬菌体透明质酸酶。第二个ORF编码功能未知的65,159分子量蛋白。通过使用含有无启动子氯霉素乙酰转移酶基因的质粒，鉴定每个ORF的推定转录和翻译控制序列。通过大肠杆菌中的T7聚合酶-启动子系统控制的hylP基因排他性表达产生了40,000道尔顿的蛋白质，其结果与hylP基因的编码能力一致。

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《Infection and immunity》 |1989年第2期|共7页
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W L Hynes; J J Ferretti;
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1. Analysis of a second bacteriophage hyaluronidase gene from Streptococcus pyogenes: evidence for a third hyaluronidase involved in extracellular enzymatic activity. [J] . W L Hynes, L Hancock, J J Ferretti Infection and immunity . 1995,第8期

机译：化脓性链球菌的第二种噬菌体透明质酸酶基因的分析：第三种透明质酸酶参与细胞外酶活性的证据。
2. Analysis of a second bacteriophage hyaluronidase gene from Streptococcus pyogenes: evidence for a third hyaluronidase involved in extracellular enzymatic activity. [J] . W L Hynes, L Hancock, J J Ferretti Infection and immunity . 1995,第8期

机译：化脓性链球菌的第二种噬菌体透明质酸酶基因的分析：第三种透明质酸酶参与细胞外酶活性的证据。
3. The hyaluronan lyase of Streptococcus pyogenes bacteriophage H4489A. [J] . Baker JR, Dong S, Pritchard DG The Biochemical Journal . 2002,第Pta1期

机译：化脓性链球菌噬菌体H4489A的透明质酸裂合酶。
4. Optimization of Recombinant Bacteriophage Lysin of Streptococcus Expression in Escherichia coli [C] . Wang Pu, Wang Xiaofeng, Chen Weiqing 2010 4th International Conference on Bioinformatics and Biomedical Engineering . 2010

机译：重组链球菌噬菌体溶菌素在大肠杆菌中表达的优化
5. Identification and Expression of the Holin Gene in Shiga-Toxigenic Escherichia coli Specific Bacteriophages and the Use of Bacteriophage Depolymerase on Stec Biofilms Formed on Food Contact Surfaces [D] . Kountoupis, Tony C. 2019

机译：Shiga-毒素大肠杆菌特异性噬菌体核心基因的鉴定和表达及在食品接触表面上形成的STEC生物膜中的噬菌体脱色酶的应用
6. Sequence analysis and expression in Escherichia coli of the hyaluronidase gene of Streptococcus pyogenes bacteriophage H4489A. [O] . W L Hynes, J J Ferretti 1989

机译：化脓性链球菌噬菌体H4489A的透明质酸酶基因的序列分析和在大肠杆菌中的表达。
7. The hyaluronan lyase of Streptococcus pyogenes bacteriophage H4489A. [O] . Baker, John R, Dong, Shengli, Pritchard, David G 2002

机译：化脓性链球菌噬菌体H4489A的透明质酸裂合酶。

Sequence analysis and expression in Escherichia coli of the hyaluronidase gene of Streptococcus pyogenes bacteriophage H4489A.

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