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首页> 外文期刊>Infection and immunity >Induction of mucosal immunity by intranasal application of a streptococcal surface protein antigen with the cholera toxin B subunit.
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Induction of mucosal immunity by intranasal application of a streptococcal surface protein antigen with the cholera toxin B subunit.

机译：通过鼻内应用链球菌表面蛋白抗原与霍乱毒素B亚基诱导粘膜免疫。

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The level and distribution of isotype-specific antibodies in various secretions and of antibody-secreting cells in corresponding lymphoid organs and tissues were compared in mice immunized with Streptococcus mutans surface protein antigen I/II (AgI/II) conjugated to the cholera toxin B subunit (CTB), given intranasally (i.n.) or intragastrically (i.g.), with or without free cholera toxin (CT) as an adjuvant. Immunization i.n. induced stronger initial antibody responses to AgI/II in both serum and saliva than immunization i.g., but salivary immunoglobulin A (IgA)-specific antibody responses to immunization about 3 months later were not increased relative to total salivary IgA concentrations. Specific antibodies induced by i.n. immunization were as widely distributed in serum, saliva, tracheal wash, gut wash, and vaginal wash as those induced by i.g. immunization. Likewise, specific antibody-secreting cells were generated in the spleen, salivary glands, intestinal lamina propria, and mesenteric and cervical lymph nodes by either route of immunization. The strongest salivary IgA antibody response was induced by AgI/II-CTB conjugate given i.n., but the addition of CT did not further enhance it. However, free CTB could effectively replace CT as an adjuvant in i.n. immunization with unconjugated AgI/II. Booster i.n. immunization with AgI/II plus either free CT or CTB induced stronger recall serum antibody responses than conjugated AgI/II-CTB with or without CT as an adjuvant. Therefore, i.n. immunization with a protein antigen and free or coupled CTB is an effective means of generating IgA antibody responses expressed at several mucosal sites where protective immunity may be beneficial.
机译：在用变形链球菌表面蛋白抗原I / II（AgI / II）结合霍乱毒素B亚基免疫的小鼠中比较了各种分泌物中同种型特异性抗体的水平和分布以及相应淋巴器官和组织中抗体分泌细胞的水平和分布（CTB），鼻内（in）或胃内（ig）给予或不给予游离霍乱毒素（CT）作为佐剂。免疫接种诱导的血清和唾液中对AgI / II的初始抗体应答均比免疫接种强，例如，唾液免疫球蛋白A（IgA）特异性抗体对免疫的应答相对于总唾液IgA浓度并未增加。 i.n.诱导的特异性抗体免疫在血清，唾液，气管洗液，肠洗液和阴道洗液中的分布与由免疫接种诱导的一样广泛。免疫。同样，通过任何一种免疫途径，在脾脏，唾液腺，固有层肠，肠系膜和颈淋巴结中都会产生特异性的抗体分泌细胞。 i.n.给予的AgI / II-CTB偶联物诱导了最强的唾液IgA抗体反应，但是添加CT并不能进一步增强它。但是，免费的CTB可以有效替代CT作为i.n.中的佐剂。用未结合的AgI / II免疫。助推器与结合或不结合CT作为佐剂的AgI / II-CTB结合，用AgI / II加游离CT或CTB免疫诱导的召回血清抗体反应更强。因此，用蛋白抗原和游离或偶联的CTB免疫是产生IgA抗体反应的有效手段，在保护性免疫可能有益的几个粘膜位点表达。

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