• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Salivary, nasal, genital, and systemic antibody responses in monkeys immunized intranasally with a bacterial protein antigen and the Cholera toxin B subunit.
【24h】

Salivary, nasal, genital, and systemic antibody responses in monkeys immunized intranasally with a bacterial protein antigen and the Cholera toxin B subunit.

机译：用细菌蛋白抗原和霍乱毒素B亚基免疫鼻内免疫猴子的唾液，鼻腔，生殖器和全身抗体反应。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Previous attempts to induce mucosal antibodies in rhesus monkeys by enteric immunization have resulted in only modest and short-lived responses, dominated by immunoglobulin M (IgM) antibodies in the plasma. In this study, two groups of rhesus monkeys were immunized intranasally three times at 2-week intervals with a bacterial protein antigen (AgI/II) either chemically coupled to or mixed with the B subunit of cholera toxin (CT), a known potent mucosal immunogen and carrier for other immunogens. Cells secreting antibodies, predominantly of the IgA isotype, to AgI/II and to CT were detected in the peripheral blood 1 week after each immunization, indicating the dissemination of IgA-secreting precursor cells through the mucosal immune system. IgG and, to a lesser extent, IgA antibodies to both proteins were induced in the plasma commencing after the second immunization. Plasma IgE concentrations and IgE antibody levels were not consistently raised during the immunization period. IgA antibodies were found in nasal and vaginal washes. Nasal IgG but not IgA antibodies showed a significant positive correlation with plasma IgG antibody levels, suggesting that they were largely derived by transudation from the circulation. Analysis of the molecular form of vaginal IgA indicated that both secretory and monomeric forms of IgA were present in various proportions. Furthermore, neither IgG nor IgA antibodies in vaginal washes were correlated with plasma antibody responses, suggesting the contribution of locally synthesized antibodies of both isotypes. Comparison of the responses between the two groups of animals showed only sporadic significant differences, indicating that intranasal immunization with AgI/II either coupled to or mixed with the B subunit of CT was equally effective at inducing generalized IgA antibody responses in the mucosal immune system and predominantly IgG antibodies in the plasma.
机译：以前通过肠溶免疫的恒河猴中诱导粘膜抗体的尝试只导致了血浆中的免疫球蛋白M（IgM）抗体所在的适度和短暂的反应。在这项研究中，将两组恒河猴以2周的间隔在2周的间隔中鼻内免疫，用细菌蛋白质抗原（AGI / II）化学偶联或与霍乱毒素（CT）的B亚基，一种已知的粘膜其他免疫原的免疫原和载体。在每次免疫后1周内在外周血中检测到分泌抗体，主要是IgA同种型的抗体，从而在外周血后检测到AGI / II和CT，表明通过粘膜免疫系统传播IGA分泌前体细胞。在较小程度上，在第二种免疫后，在较小程度上诱导两种蛋白质的IgA抗体。在免疫时间内不始终升高等离子体IgE浓度和IgE抗体水平。在鼻腔和阴道洗涤中发现IgA抗体。鼻IgG但不是IgA抗体显示出与血浆IgG抗体水平显着的阳性相关性，这表明它们主要通过循环的转换来源于衍生。阴道IgA分子形式的分析表明，分泌物和单体形式的IgA以各种比例存在。此外，阴道洗涤中的IgG和IgA抗体都不与血浆抗体反应相关，表明两种同种型的局部合成抗体的贡献。两组动物之间的反应的比较仅表明散发性显着差异，表明用CT的CT的B亚基与AGI / II偶联的鼻内免疫同等有效诱导粘膜免疫系统中的广义IgA抗体反应。主要是血浆中的IgG抗体。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号