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首页> 外文期刊>Infection and immunity >Leishmania pifanoi amastigote antigens protect mice against cutaneous leishmaniasis.
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Leishmania pifanoi amastigote antigens protect mice against cutaneous leishmaniasis.

机译：皮氏利什曼原虫鞭毛体抗原保护小鼠免于皮肤利什曼病。

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In the search for a leishmaniasis vaccine, extensive studies have been carried out with promastigote (insect stage) molecules. Information in this regard on amastigote (mammalian host stage) molecules is limited. To investigate host immune responses to Leishmania amastigote antigens, we purified three stage-specific antigens (A2, P4, and P8) from in vitro-cultivated amastigotes of Leishmania pifanoi by using immunoaffinity chromatography. We found that with Corynebacterium parvum as an adjuvant, three intraperitoneal injections of 5 micrograms of P4 or P8 antigen provided partial to complete protection of BALB/c mice challenged with 10(5) to 10(7) L. pifanoi promastigotes. These immunized mice developed significantly smaller or no lesions and exhibited a 39- to 1.6 x 10(5)-fold reduction of lesion parasite burden after 15 to 20 weeks of infection. In addition, P8 immunization resulted in complete protection against L. amazonensis infection of CBA/J mice and partial protection of BALB/c mice, suggesting that this antigen provided cross-species protection of mice with different H-2 haplotypes. At different stages during infection, vaccinated mice exhibited profound proliferative responses to parasite antigens and increased levels of gamma interferon production, suggesting that a Th1 cell-mediated immune response is associated with the resistance in these mice. Taken together, the data in this report indicate the vaccine potential of amastigote-derived antigens.
机译：在寻找利什曼病疫苗时，已对前鞭毛体（昆虫阶段）分子进行了广泛的研究。在这方面，关于鞭毛体（哺乳动物宿主阶段）分子的信息是有限的。为了研究宿主对利什曼原虫鞭毛虫抗原的免疫应答，我们使用免疫亲和层析从体外培养的利什曼原虫pifanoi的变形虫纯化了三个阶段特异性抗原（A2，P4和P8）。我们发现，以小棒杆菌为佐剂，腹膜内注射5微克P4或P8抗原可提供3次部分腹腔注射，以完全保护受到10（5）至10（7）L. pifanoi前鞭毛体攻击的BALB / c小鼠。这些经过免疫的小鼠在感染15至20周后，病灶明显变小或没有病变，并减少了39至1.6 x 10（5）倍的病原体寄生虫负担。此外，P8免疫可完全防御C.A / J小鼠对亚马逊乳杆菌的感染，并对BALB / c小鼠具有部分保护作用，表明该抗原为具有不同H-2单倍型的小鼠提供了跨物种保护。在感染过程的不同阶段，接种疫苗的小鼠对寄生虫抗原表现出深远的增生反应，并且γ干扰素的产生水平增加，这表明Th1细胞介导的免疫反应与这些小鼠的耐药性有关。综上所述，本报告中的数据表明了由鞭毛体来源的抗原的疫苗潜力。

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