• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Transfer of Antigen-Pulsed Dendritic Cells Induces Specific T-Cell Proliferation and a Therapeutic Effect against Long-Term Helicobacter pylori Infection in Mice
【24h】

Transfer of Antigen-Pulsed Dendritic Cells Induces Specific T-Cell Proliferation and a Therapeutic Effect against Long-Term Helicobacter pylori Infection in Mice

机译：抗原脉冲树突状细胞的转移诱导特异性T细胞增殖和对小鼠长期幽门螺杆菌感染的治疗作用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Helicobacter pylori causes persistent infection of the stomach and results in chronic gastritis and peptic ulcers. Jaws II cells, derived from mouse bone marrow, were pulsed with live or formalin-killed or whole-cell sonicates (WCS) of H. pylori. Representative cell surface molecules were expressed at substantial levels on Jaws II cells, indicating that appropriate maturation of the cells was achieved with the three H. pylori antigens without any significant differences. H. pylori WCS-pulsed Jaws II cells secreted a significant amount of tumor necrosis factor alpha into the culture supernatant. The na?ve T cells exposed to the WCS-pulsed Jaws II cells showed significant proliferation and gamma interferon (IFN-γ) and interleukin-10 (IL-10) production in vitro. A 2-log reduction in the number of colonizing bacteria was observed in the mice treated with the WCS-pulsed Jaws II cells; however, no significant reductions were achieved in mice treated with Jaws II cells pulsed with other H. pylori antigens. Up-regulated production of IFN-γ and IL-10 was observed in the stomachs of the mice treated with the WCS-pulsed Jaws II cells, which is consistent with the result obtained in vitro. There were no differences in gastritis scores or H. pylori-specific antibody titers among the mice treated with Jaws II cells pulsed with the three different H. pylori antigens. The results suggest that Th1 cell-mediated immunity in combination with Th2 cell-mediated immunity plays a role in reducing colonizing bacterial numbers in mice with chronic H. pylori infections.
机译：幽门螺杆菌引起胃的持续感染，并导致慢性胃炎和消化性溃疡。用活的或福尔马林杀死的或H的全细胞超声（WCS）对源自小鼠骨髓的Jaws II细胞进行脉冲处理。幽门炎。代表性的细胞表面分子在Jaws II细胞上大量表达，表明通过三个 H实现了细胞的适当成熟。幽门螺杆菌抗原无明显差异。 H。幽门螺杆菌WCS脉冲的Jaws II细胞在培养上清液中分泌了大量的肿瘤坏死因子α。暴露于WCS脉冲的Jaws II细胞的幼稚T细胞在体外具有明显的增殖和γ-干扰素（IFN-γ）和白介素10（IL-10）的产生。在用WCS脉冲的Jaws II细胞处理的小鼠中，观察到定殖细菌数量减少了2个对数;然而，在用其他 H脉冲刺激的Jaws II细胞处理的小鼠中，未实现明显降低。幽门螺杆菌抗原。在用WCS脉冲的Jaws II细胞处理的小鼠的胃中观察到IFN-γ和IL-10的上调产生，这与体外获得的结果一致。胃炎评分或 H没有差异。在用三种不同的 H脉冲的Jaws II细胞处理的小鼠中，幽门螺杆菌特异性抗体滴度。幽门螺杆菌抗原。结果表明，Th1细胞介导的免疫与Th2细胞介导的免疫相结合，在减少慢性 H小鼠的定殖细菌数量中发挥了作用。幽门螺杆菌感染。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号