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首页> 外文期刊>Infection and immunity >Immunization with Native or Recombinant Streptococcus pneumoniae Neuraminidase Affords Protection in the Chinchilla Otitis Media Model
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Immunization with Native or Recombinant Streptococcus pneumoniae Neuraminidase Affords Protection in the Chinchilla Otitis Media Model

机译：在龙猫中耳炎模型中用天然或重组肺炎链球菌神经氨酸酶Affords保护免疫

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Streptococcus pneumoniae neuraminidase has been implicated as a virulence factor in the pathogenesis of pneumococcal otitis media. In this study, native neuraminidase was partially purified from cultures of S. pneumoniae by serial chromatography with DEAE-Sepharose and Sephacryl S-200. Recombinant neuraminidase, a 3,038-bp fragment of the neuraminidase A (nanA) gene, was cloned into the pET-28b vector and then expressed at high levels in Escherichia coli. Chinchillas were immunized subcutaneously with either the gel-purified native or recombinant neuraminidase, and all responded with elevated titers of antineuraminidase antibody in serum. Immunization with neuraminidase resulted in a significant reduction in nasopharyngeal colonization as well as in the incidence of otitis media with effusion. These data demonstrate for the first time that neuraminidase affords protection against S. pneumoniae nasopharyngeal colonization and experimental otitis media.
机译：肺炎链球菌神经氨酸酶被认为是肺炎球菌性中耳炎发病机理中的一种致病因子。在这项研究中，天然神经氨酸酶是从 S培养物中部分纯化的。用DEAE-Sepharose和Sephacryl S-200进行系列色谱分析肺炎。将重组神经氨酸酶（神经氨酸酶A（ nanA ）基因的3,038 bp片段）克隆到pET-28b载体中，然后在大肠杆菌中高水平表达。用凝胶纯化的天然或重组神经氨酸酶对龙猫进行皮下免疫，所有这些抗体均以升高的血清抗神经氨酸酶抗体的滴度应答。用神经氨酸酶免疫导致鼻咽部定植显着减少以及积液性中耳炎的发生。这些数据首次证明神经氨酸酶提供针对 S的保护。肺炎性鼻咽部定植和实验性中耳炎。

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