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首页> 外文期刊>Infection and immunity >Role of Yops and Adhesins in Resistance of Yersinia enterocolitica to Phagocytosis
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Role of Yops and Adhesins in Resistance of Yersinia enterocolitica to Phagocytosis

机译：Yop和粘附素在小肠结肠炎耶尔森氏菌对吞噬作用的抗性中的作用

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Yersinia enterocolitica is a pathogen endowed with two adhesins, Inv and YadA, and with the Ysc type III secretion system, which allows extracellular adherent bacteria to inject Yop effectors into the cytosol of animal target cells. We tested the influence of all of these virulence determinants on opsonic and nonopsonic phagocytosis by PU5-1.8 and J774 mouse macrophages, as well as by human polymorphonuclear leukocytes (PMNs). The adhesins contributed to phagocytosis in the absence of opsonins but not in the presence of opsonins. In agreement with previous results, YadA counteracted opsonization. In every instance, the Ysc-Yop system conferred a significant level of resistance to phagocytosis. Nonopsonized single-mutant bacteria lacking either YopE, -H, -T, or -O were phagocytosed significantly more by J774 cells and by PMNs. Opsonized bacteria were phagocytosed more than nonopsonized bacteria, and mutant bacteria lacking either YopH, -T, or -O were phagocytosed significantly more by J774 cells and by PMNs than were wild-type (WT) bacteria. Opsonized mutants lacking only YopE were phagocytosed significantly more than were WT bacteria by PMNs but not by J774 cells. Thus, YopH, -T, and -O were involved in all of the phagocytic processes studied here but YopE did not play a clear role in guarding against opsonic phagocytosis by J774. Mutants lacking YopP and YopM were, in every instance, as resistant as WT bacteria. Overexpression of YopE, -H, -T, or -O alone did not confer resistance to phagocytosis, although it affected the cytoskeleton. These results show that YopH, YopT, YopO, and, in some instances, YopE act synergistically to increase the resistance of Y. enterocolitica to phagocytosis by macrophages and PMNs.
机译：小肠结肠炎耶尔森菌是一种病原体，具有两种粘附素Inv和YadA，并具有Ysc III型分泌系统，该系统可使细胞外粘附细菌将Yop效应子注入动物靶细胞的细胞质中。我们测试了所有这些毒力决定因素对PU5-1.8和J774小鼠巨噬细胞以及人类多形核白细胞（PMN）的调理和非调理吞噬作用的影响。在没有调理素的情况下，粘附素有助于吞噬作用，但是在调理素的情况下则没有。与以前的结果一致，YadA反对调理人参。在每种情况下，Ysc-Yop系统都赋予了显着水平的抗吞噬能力。缺乏YopE，-H，-T或-O的非调理单突变细菌被J774细胞和PMN吞噬的更多。与非调理细菌相比，调理细菌的吞噬能力更高，而与野生型（WT）细菌相比，J774细胞和PMN吞噬缺少YopH，-T或-O的突变细菌的能力明显更高。仅缺少YopE的调理突变体比PM细菌对WT细菌的吞噬作用明显增强，而对J774细胞的吞噬作用却显着提高。因此，YopH，-T和-O参与了本文研究的所有吞噬过程，但YopE在J774的抗调理吞噬作用中没有明确的作用。在每种情况下，缺乏YopP和YopM的突变体与WT细菌一样具有抗性。单独的YopE，-H，-T或-O的过度表达虽然影响细胞骨架，但并未赋予对吞噬作用的抗性。这些结果表明，YopH，YopT，YopO以及在某些情况下，YopE具有协同作用，以提高 Y的抗性。肠巨噬细胞通过巨噬细胞和PMN吞噬。

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