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首页> 外文期刊>Infection and immunity >The hemagglutinin gene A (hagA) of Porphyromonas gingivalis 381 contains four large, contiguous, direct repeats.
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The hemagglutinin gene A (hagA) of Porphyromonas gingivalis 381 contains four large, contiguous, direct repeats.

机译：牙龈卟啉单胞菌381的血凝素基因A（hagA）包含四个连续的大重复序列。

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Porphyromonas gingivalis is a gram-negative anaerobic bacterial species strongly associated with adult periodontitis. One of its distinguishing characteristics and putative virulence properties is the ability to agglutinate erythrocytes. We have previously reported the cloning of multiple hemagglutinin genes from P. gingivalis 381. Subsequent sequencing of clone ST 2 revealed that the cloned fragment contained only an internal portion of the gene which lacked both start and stop codons. We here report the cloning and sequencing of the entire gene, designated hagA, as well as its relationship to other genes of this species. By use of inverse PCR technology and the construction of several additional genomic libraries, the complete open reading frame of hagA was found to be 7,887 bp in length, encoding a protein of 2,628 amino acids with a molecular mass of 283.3 kDa, which is among the largest genes ever cloned from a prokaryote to date. Within its open reading frame, four large, contiguous, direct repeats (varying from 1,318 to 1,368 bp) were identified. The repeat unit (HArep), which is assumed to contain the hemagglutinin domain, is also present in other recently reported protease and hemagglutinin genes in P. gingivalis. Thus, we propose that hagA and the other genes which share the HArep sequence form a multigene family with hagA as a central member.
机译：牙龈卟啉单胞菌是与成人牙周炎密切相关的革兰氏阴性厌氧细菌。其区别特征和假定的毒力特性之一是凝集红细胞的能力。我们先前曾报道过从牙龈卟啉单胞菌381中克隆多个血凝素基因。随后对克隆ST 2的测序表明，克隆的片段仅包含该基因的内部部分，该部分既缺乏起始密码子又没有终止密码子。我们在这里报告了命名为hagA的整个基因的克隆和测序，及其与该物种其他基因的关系。通过使用反向PCR技术和几个其他基因组文库的构建，发现hagA的完整开放阅读框长度为7887 bp，编码2628个氨基酸的蛋白质，分子量为283.3 kDa，属于迄今为止从原核生物中克隆的最大基因。在其开放阅读框内，鉴定出四个大的，连续的直接重复序列（从1,318 bp到1,368 bp）。假定包含血凝素结构域的重复单元（HArep）也存在于牙龈卟啉单胞菌的其他最近报道的蛋白酶和血凝素基因中。因此，我们建议hagA和其他共享HArep序列的基因形成一个以hagA为中心成员的多基因家族。

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