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首页> 外文期刊>Infection and immunity >Role of gamma interferon in natural clearance of Bordetella pertussis infection.
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Role of gamma interferon in natural clearance of Bordetella pertussis infection.

机译：γ干扰素在百日咳博德特氏菌感染自然清除中的作用。

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Using a mouse model of Bordetella pertussis infection, we have analyzed the role of gamma interferon (IFN-gamma) in bacterial clearance from the respiratory tract. Adult BALB/c mice began to clear a respiratory infection within 3 weeks postinfection, with complete resolution of infection 6 to 8 weeks postinfection. In contrast, neither adult SCID mice (which lack mature B and T lymphocytes) nor adult nude mice (which lack mature T lymphocytes) controlled B. pertussis infection, and both strains died within 3 to 5 weeks postinfection. Short-term T-cell lines generated from the draining lymph nodes of the lungs of infected BALB/c mice were found to be CD4+ and produced IFN-gamma but no detectable interleukin-4. Analyses of IFN-gamma mRNA induction in the lungs of mice following B. pertussis infection showed that in both BALB/c and C57BL/6 mice, IFN-gamma mRNA levels increased sharply by 1 week postinfection and then subsequently declined. Further exploration of a potential role for IFN-gamma demonstrated that infection of adult BALB/c mice depleted of IFN-gamma in vivo with anti-IFN-gamma monoclonal antibodies resulted in greater numbers of bacteria recovered from the lungs than in infected, control BALB/c mice, although IFN-gamma-depleted mice could subsequently clear the infection. Infection of mice which have a disrupted IFN-gamma gene resulted in bacterial clearance with a time course similar to those seen with IFN-gamma-depleted mice. These results indicate that IFN-gamma plays a role in controlling B. pertussis infection.
机译：使用百日咳博德特氏菌感染的小鼠模型，我们分析了γ-干扰素（IFN-γ）在从呼吸道清除细菌中的作用。成年BALB / c小鼠在感染后3周内开始清除呼吸道感染，感染后6至8​​周完全消退。相反，成年SCID小鼠（缺乏成熟的B和T淋巴细胞）和成年裸鼠（没有成熟的T淋巴细胞）均不能控制百日咳博德特氏菌感染，并且两种菌株都在感染后3至5周内死亡。发现从感染的BALB / c小鼠的肺部引流淋巴结产生的短期T细胞系为CD4 +，并产生IFN-γ，但未检测到白介素-4。百日咳博德特氏菌感染后小鼠肺中IFN-γmRNA的诱导分析表明，在BALB / c和C57BL / 6小鼠中，感染后1周，IFN-γmRNA的含量均急剧上升，然后下降。进一步探索IFN-γ的潜在作用表明，体内抗IFN-γ单克隆抗体感染了体内耗竭IFN-γ的成年BALB / c小鼠，与感染的对照BALB相比，从肺中回收的细菌数量更多/ c小鼠，尽管IFN-γ耗竭的小鼠可以随后清除感染。 IFN-γ基因被破坏的小鼠的感染导致细菌清除，其时间过程与IFN-γ耗竭的小鼠相似。这些结果表明，IFN-γ在控制百日咳博德特氏菌感染中起作用。

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