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首页> 外文期刊>Infection and immunity >Synthesis, stability, and subcellular distribution of major histocompatibility complex class II molecules in Langerhans cells infected with Leishmania major.
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Synthesis, stability, and subcellular distribution of major histocompatibility complex class II molecules in Langerhans cells infected with Leishmania major.

机译：主要组织相容性复合物II类分子在感染利什曼原虫的朗格汉斯细胞中的合成，稳定性和亚细胞分布。

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Protozoan parasites of the genus Leishmania exist as obligatory intracellular amastigotes and invade macrophages and Langerhans cells, the dendritic cells of the skin. Langerhans cells are much more efficient in presenting Leishmania major antigen to T cells than macrophages are and have the unique ability to retain parasite antigen in immunogenic form for prolonged periods. To analyze the mechanisms that are responsible for this potency, we defined the synthesis, turnover, conformation, and localization of major histocompatibility complex (MHC) class II molecules in Langerhans cells. Hence, Langerhans cells were pulse-labeled; immunoprecipitation of MHC class II molecules and gel electrophoresis followed. In addition, the subcellular distribution of MHC class II molecules in L. major-infected Langerhans cells was analyzed by confocal microscopy. The results show that (i) newly synthesized MHC class II molecules are required for L. major antigen presentation by Langerhans cells, (ii) MHC class II-peptide complexes in Langerhans cells are long-lived, (iii) phagocytosis of L. major modulates MHC class II biosynthesis by reducing its downregulation during Langerhans cell differentiation, and (iv) newly synthesized MHC class II molecules are associated with the parasitophorous vacuole of infected Langerhans cells. These findings support the conclusion that the traits of MHC class II expression correspond to the highly specialized functions of Langerhans cells in the immunoregulation of cutaneous leishmaniasis.
机译：利什曼原虫属的原生动物寄生虫以必需的胞内变形虫存在，并侵入巨噬细胞和朗格汉斯细胞，即皮肤的树突状细胞。与巨噬细胞相比，朗格汉斯细胞将利什曼原虫主要抗原呈递给T细胞的效率要高得多，并且具有将寄生虫抗原以免疫原性形式长期保留的独特能力。为了分析造成这种效力的机制，我们定义了朗格汉斯细胞中主要组织相容性复合体（MHC）II类分子的合成，更新，构象和定位。因此，朗格汉斯细胞被脉冲标记。随后进行MHC II类分子的免疫沉淀和凝胶电泳。另外，通过共聚焦显微镜分析了在大肠埃希氏菌感染的Langerhans细胞中MHC II类分子的亚细胞分布。结果表明（i）Langerhans细胞呈递L.major抗原需要新合成的MHC II类分子;（ii）Langerhans细胞中MHC II类-肽复合物是长寿的;（iii）L.major的吞噬作用通过降低朗格汉斯细胞分化过程中的下调来调节MHC II类生物合成，并且（iv）新合成的II类MHC分子与被感染的朗格汉斯细胞的寄生虫空泡相关。这些发现支持以下结论：MHC II类表达的特征与朗格汉斯细胞在皮肤利什曼病的免疫调节中的高度专门化功能相对应。

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