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Carrier Properties of a Protein Derived from Outer Membrane Protein A of Klebsiella pneumoniae

机译：肺炎克雷伯氏菌外膜蛋白A衍生蛋白的载体性质

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We have recently cloned a new protein, recombinant P40 (rP40). When tested in vivo after conjugation to a B-cell epitope, rP40 induces an important antibody response without the need for adjuvant. To characterize its potency, this carrier protein was coupled to a peptide derived from respiratory syncytial virus attachment G protein (G1′). After immunization of mice with the rP40-G1′ conjugate, strong antipeptide antibodies were detected, whereas peptide alone was not immunogenic. To emphasize the carrier properties of rP40, a polysaccharide derived from Haemophilus influenzae type b (Hib) was coupled to it. Immunoglobulin G responses against the Hib polysaccharide were observed after coupling to rP40. Interestingly, an antipeptide antibody response was observed despite preexisting anti-rP40 antibodies generated by preimmunization with rP40. In addition, rP40 compares well with the reference carrier protein, tetanus toxoid (TT), since antibody responses of equal intensity were observed when a peptide or a polysaccharide was coupled to TT and rP40. Moreover, rP40 had advantages compared to TT; e.g., it induced a mixed Th1/Th2 response, whereas TT induced only a Th2 profile. Together, the results indicate that rP40 is a novel carrier protein with potential for use as an alternative carrier for human vaccination.
机译：我们最近克隆了一种新蛋白，重组P40（rP40）。当与B细胞表位结合后在体内进行测试时，rP40诱导了重要的抗体反应，而无需佐剂。为了表征其效力，将该载体蛋白与衍生自呼吸道合胞病毒附着G蛋白（G1'）的肽偶联。用rP40-G1'偶联物免疫小鼠后，检测到强抗肽抗体，而单独的肽没有免疫原性。为了强调rP40的载体特性，将来源于流感嗜血杆菌b型（Hib）的多糖与之偶联。偶联rP40后，观察到针对Hib多糖的免疫球蛋白G反应。有趣的是，尽管预先存在用rP40免疫产生的抗rP40抗体，但仍观察到抗肽抗体反应。此外，rP40与参考载体蛋白破伤风类毒素（TT）的比较好，因为当肽或多糖与TT和rP40偶联时，观察到强度相同的抗体反应。此外，rP40与TT相比具有优势。例如，它诱导了混合的Th1 / Th2反应，而TT仅诱导了Th2谱。在一起，结果表明rP40是一种新型的载体蛋白，有潜力用作人类疫苗的替代载体。

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