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首页> 外文期刊>Infection and immunity >Mycobacterium leprae produces extracellular homologs of the antigen 85 complex.
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Mycobacterium leprae produces extracellular homologs of the antigen 85 complex.

机译：麻风分枝杆菌产生抗原85复合物的细胞外同源物。

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The antigen 85 complex is a set of at least three closely related secreted proteins (85A, 85B, and 85C) of 30 to 32 kDa produced by Mycobacterium tuberculosis and other mycobacteria. Their prominence in Mycobacterium leprae, the one obligate intracellular pathogen of the genus, had been assumed on the basis of immunological evidence and proof of the existence of the gene encoding the 85B protein of the complex. We have now observed the production of this family of proteins by M. leprae through analysis of various fractions by Western blotting (immunoblotting) with monospecific rabbit antisera raised against the individual Mycobacterium bovis BCG 85A, 85B, and 85C proteins. A predominant cross-reactive band with an apparent molecular mass of 30 kDa was detected in extracts of nondisrupted whole M. leprae and in soluble fractions prepared from the tissues of M. leprae-infected armadillos. Further studies of the subcellular distribution of this protein within the bacterium confirmed that it is secreted by the organism, an observation that explains past difficulties in detecting the antigen 85 complex in M. leprae. Confirmation that the M. leprae product is a member of the antigen 85 complex was obtained by comparison of peptide fingerprints with those from the BCG product. The pattern of reactivity of the M. leprae antigen 85 complex with anti-M. bovis BCG 85B serum, as well as two-dimensional electrophoresis, established that the 85B component was the predominant member of the complex in M. leprae. The fibronectin-binding capacity of the M. leprae and BCG 85 complexes was reinvestigated by new approaches and is questioned. Nevertheless, the results obtained with the native proteins reinforce previous reports, derived primarily from the use of homologous proteins, that the antigen 85 complex is one of the dominant protein immunogens of the leprosy bacillus.
机译：抗原85复合物是由结核分枝杆菌和其他分枝杆菌产生的至少三种紧密相关的分泌蛋白（85A，85B和85C）的集合，其为30至32kDa。根据免疫学证据和编码该复合物85B蛋白基因的存在，推测它们在麻风分枝杆菌（一种专属的细胞内病原体）中的重要性。现在，我们已经通过麻疯分枝杆菌通过针对单个牛分枝杆菌BCG 85A，85B和85C蛋白产生的单特异性兔抗血清的蛋白质印迹分析（免疫印迹）分析了各种组分，从而观察到该蛋白家族的生产。在未破裂的完整麻风支原体的提取物中和从麻风支原体感染的犰狳组织制备的可溶性级分中检测到主要的表观分子量为30 kDa的交叉反应带。对该蛋白在细菌内的亚细胞分布的进一步研究证实了它是由生物体分泌的，这一发现解释了过去检测麻风分枝杆菌中抗原85复合物的困难。通过比较肽指纹图谱和BCG产物的指纹图谱，可以确认麻风杆菌产品是抗原85复合物的成员。麻风分枝杆菌抗原85复合物与抗M的反应性模式。牛BCG 85B血清和二维电泳确定了85B成分是麻风分枝杆菌中复合物的主要成分。麻风分枝杆菌和BCG 85复合物的纤连蛋白结合能力已通过新方法进行了重新调查，并受到质疑。尽管如此，使用天然蛋白获得的结果加强了以前的报道，这些报道主要源于使用同源蛋白，即抗原85复合物是麻风杆菌的主要蛋白免疫原之一。

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