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首页> 外文期刊>Infection and immunity >Adherence, fibronectin binding, and induction of cytoskeleton reorganization in cultured human cells by Mycoplasma penetrans.
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Adherence, fibronectin binding, and induction of cytoskeleton reorganization in cultured human cells by Mycoplasma penetrans.

机译：支原体支原体在培养的人细胞中的粘附，纤连蛋白结合和诱导的细胞骨架重组。

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Mycoplasma penetrans adhered to cultured human cells, forming clusters that localized to specific areas of the host cell surface. Adherence and cluster formation were inhibited by anti-M. penetrans antibodies, suggesting the involvement of specific adhesin-receptor interactions. Ultrastructural studies showed that after 2 h of infection, mycoplasmas attach to and penetrate the host cell surface. M. penetrans bound selectively to immobilized fibronectin, an interaction which was not inhibited by a 70-kDa fragment containing a heparin-gelatin-binding domain of fibronectin, other matrix glycoproteins, or an RGD tripeptide, suggesting the recognition of other specific binding sites on the fibronectin molecule. A ca. 65-kDa fibronectin-binding protein of M. penetrans was eluted following Sepharose-fibronectin affinity chromatography. Confocal, light, and immunofluorescence microscopy demonstrated that the interaction of M. penetrans with target cells triggers a signal that causes recruitment of several cytoskeletal components, including tubulin and alpha-actinin, and aggregation of phosphorylated proteins. Detergent-soluble mycoplasma proteins with apparent molecular masses of 18, 28, 32, 36, 39, and 41 kDa selectively bound to glutaraldehyde-fixed HEp-2 cells. Our findings offer new insights into understanding the interaction of this human mycoplasma with host target cells.
机译：支原体支原体粘附在培养的人类细胞上，形成簇，位于宿主细胞表面的特定区域。粘附和簇形成被抗-M抑制。 penetrans抗体，提示特定的粘附素-受体相互作用。超微结构研究表明，感染2小时后，支原体附着并穿透宿主细胞表面。 penetrans选择性结合固定的纤连蛋白，这种相互作用不受包含纤连蛋白，其他基质糖蛋白或RGD三肽的肝素-明胶结合结构域的70 kDa片段的抑制，表明存在其他特异性结合位点的识别纤连蛋白分子。大约用琼脂糖-纤连蛋白亲和色谱法洗脱戊型支原体的65-kDa纤连蛋白结合蛋白。共聚焦，光和免疫荧光显微镜检查表明，penetrans支原体与靶细胞的相互作用触发了一个信号，该信号引起几种细胞骨架成分的募集，包括微管蛋白和α-肌动蛋白，以及磷酸化蛋白的聚集。表观分子量为18、28、32、36、39和41 kDa的去污剂可溶性支原体蛋白选择性结合戊二醛固定的HEp-2细胞。我们的发现为了解人类支原体与宿主靶细胞的相互作用提供了新的见解。

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