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首页> 外文期刊>Infection and immunity >Evaluation of Immunoglobulin A1 (IgA1) Protease and IgA1 Protease-Inhibitory Activity in Human Female Genital Infection with Neisseria gonorrhoeae
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Evaluation of Immunoglobulin A1 (IgA1) Protease and IgA1 Protease-Inhibitory Activity in Human Female Genital Infection with Neisseria gonorrhoeae

机译：淋病奈瑟氏菌感染女性女性的免疫球蛋白A1（IgA1）蛋白酶和IgA1蛋白酶抑制活性的评估

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Immunoglobulin A1 (IgA1) protease, an enzyme that selectively cleaves human IgA1, may be a virulence factor for pathogenic organisms such as Neisseria gonorrhoeae. Host protection from the effects of IgA1 protease includes antibody-mediated inhibition of IgA1 protease activity, and it is believed that the relative balance between IgA1 protease and inhibitory antibodies contributes to the pathogenesis of disease caused by IgA1 protease-producing organisms. We have examined the levels of these two opposing factors in genital tract secretions and sera from women with uncomplicated infection withN. gonorrhoeae. When IgA1 in cervical mucus was examined by Western blotting, no evidence of cleavage fragments characteristic of IgA1 protease activity was seen in gonococcus-infected or control patients. Cleavage fragments typical of IgA1 protease were detected, however, after the addition of exogenous IgA1 protease to cervical mucus. Degraded IgA1 was detected in some vaginal wash samples, but the fragment pattern was not typical of IgA1 protease activity. AllN. gonorrhoeae isolates from the infected patients produced IgA1 protease in vitro. All but two serum samples and 16 of 65 cervical mucus samples displayed inhibitory activity against gonococcal IgA1 protease, but there was no significant difference in the level of inhibitory activity between gonococcus-infected and noninfected patients in either cervical mucus or serum. There was no difference in the levels of IgA1 protease-inhibitory activity in serum or cervical mucus collected from patients at recruitment and 2 weeks later. These results suggest that cleavage of IgA1 by gonococcal IgA1 protease within the lumen of the female lower genital tract is unlikely to be a significant factor in the pathogenesis of infections by N. gonorrhoeae.
机译：免疫球蛋白A1（IgA1）蛋白酶是一种选择性裂解人IgA1的酶，可能是淋病奈瑟氏球菌等致病生物的致病因子。宿主免受IgA1蛋白酶影响的保护包括抗体介导的IgA1蛋白酶活性的抑制，并且据信IgA1蛋白酶和抑制性抗体之间的相对平衡有助于由产生IgA1蛋白酶的生物引起的疾病的发病机理。我们已经检查了未感染Nem的女性生殖道分泌物和血清中这两个对立因素的水平。淋病菌。当通过Western印迹检查宫颈粘液中的IgA1时，在淋球菌感染或对照患者中没有发现具有IgA1蛋白酶活性特征的切割片段的证据。但是，在向宫颈粘液中添加外源IgA1蛋白酶后，检测到了典型的IgA1蛋白酶裂解片段。在一些阴道洗液样品中检测到降解的IgA1，但该片段模式不是IgA1蛋白酶活性的典型特征。全部 N。感染患者的淋球菌分离株在体外产生了IgA1蛋白酶。除了两个血清样本和65个宫颈粘液样本中的16个以外，所有样本均显示出对淋球菌IgA1蛋白酶的抑制活性，但是在宫颈粘液或血清中，淋球菌感染和未感染的患者的抑制活性水平没有显着差异。在招募时和2周后从患者收集的血清或宫颈粘液中，IgA1蛋白酶抑制活性水平没有差异。这些结果表明，淋巴球菌IgA1蛋白酶在女性下生殖道管腔内切割IgA1不太可能是 N感染发病机理的重要因素。淋病菌。

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