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首页> 外文期刊>Infection and immunity >Expression of the Yersinia pestis capsular antigen (F1 antigen) on the surface of an aroA mutant of Salmonella typhimurium induces high levels of protection against plague.
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Expression of the Yersinia pestis capsular antigen (F1 antigen) on the surface of an aroA mutant of Salmonella typhimurium induces high levels of protection against plague.

机译：鼠伤寒沙门氏菌aroA突变体表面上的鼠疫耶尔森氏菌荚膜抗原（F1抗原）的表达诱导了对鼠疫的高水平保护。

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摘要

The caf operon from Yersinia pestis encoding the structural subunit (caf1), the molecular chaperone (caf1M), the outer membrane anchor (caf1A), and the regulatory protein (caf1R) was cloned into Salmonella typhimurium SL3261 aroA. The recombinant Salmonella organisms were encapsulated when cultured at 37 degrees C but not when cultured at 28 degrees C. Oral inoculation of mice with the recombinant Salmonella induced predominantly an immunoglobulin G2a response to F1 antigen, and isolated T cells showed a recall response to soluble or Salmonella-associated F1 antigen. Mice immunized with S. typhimurium SL3261 aroA expressing F1 antigen intracellularly developed lower antibody responses to F1 antigen and showed a T-cell recall response only to Salmonella-associated F1 antigen. Mice immunized orally with two doses of the recombinant Salmonella which expressed F1 antigen on the surface were protected against 10(7) 50% lethal doses (LD50) of virulent Y. pestis given by the subcutaneous route of challenge, whereas mice immunized with the recombinant Salmonella expressing F1 antigen intracellularly were only partially protected against 10(5) LD50 of Y. pestis.
机译：将鼠疫耶尔森氏菌的caf操纵子编码结构亚基（caf1），分子伴侣（caf1M），外膜锚（caf1A）和调节蛋白（caf1R）克隆到鼠伤寒沙门氏菌SL3261 aroA中。重组沙门氏菌生物体在37摄氏度培养时被封装，而在28摄氏度培养时则没有。用重组沙门氏菌口服接种的小鼠主要诱导对F1抗原的免疫球蛋白G2a应答，而分离的T细胞对可溶性或可溶性的T细胞显示召回应答沙门氏菌相关的F1抗原。用表达F1抗原的鼠伤寒沙门氏菌SL3261 aroA免疫的小鼠在细胞内产生了对F1抗原的较低抗体应答，并且仅对沙门氏菌相关F1抗原表现出T细胞召回应答。口服免疫接种了两剂在表面表达F1抗原的重组沙门氏菌的小鼠免受了皮下攻击途径给予的10（7）50％致命剂量的致病性鼠疫杆菌（LD50）的攻击，而用重组疫苗免疫的小鼠沙门氏菌在细胞内表达F1抗原仅部分保护免受鼠疫耶尔森氏菌的10（5）LD50。

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