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首页> 外文期刊>Infection and immunity >Production of β-Defensin Antimicrobial Peptides by the Oral Mucosa and Salivary Glands
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Production of β-Defensin Antimicrobial Peptides by the Oral Mucosa and Salivary Glands

机译：口腔粘膜和唾液腺产生β-防御素抗菌肽

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β-Defensins are cationic peptides with broad-spectrum antimicrobial activity that are produced by epithelia at mucosal surfaces. Two human β-defensins, HBD-1 and HBD-2, were discovered in 1995 and 1997, respectively. However, little is known about the expression of HBD-1 or HBD-2 in tissues of the oral cavity and whether these proteins are secreted. In this study, we characterized the expression of HBD-1 and HBD-2 mRNAs within the major salivary glands, tongue, gingiva, and buccal mucosa and detected β-defensin peptides in salivary secretions. Defensin mRNA expression was quantitated by RNase protection assays. HBD-1 mRNA expression was detected in the gingiva, parotid gland, buccal mucosa, and tongue. Expression of HBD-2 mRNA was detected only in the gingival mucosa and was most abundant in tissues with associated inflammation. To test whether β-defensin expression was inducible, gingival keratinocyte cell cultures were treated with interleukin-1β (IL-1β) or bacterial lipopolysaccharide (LPS) for 24 h. HBD-2 expression increased ～16-fold with IL-1β treatment and ～5-fold in the presence of LPS. Western immunoblotting, liquid chromatography, and mass spectrometry were used to identify the HBD-1 and HBD-2 peptides in human saliva. Human β-defensins are expressed in oral tissues, and the proteins are secreted in saliva; HBD-1 expression was constitutive, while HBD-2 expression was induced by IL-1β and LPS. Human β-defensins may play an important role in the innate defenses against oral microorganisms.
机译：β-防御素是由粘膜表面上皮细胞产生的具有广谱抗菌活性的阳离子肽。分别在1995年和1997年发现了两种人类β-防御素HBD-1和HBD-2。但是，关于口腔组织中的HBD-1或HBD-2的表达以及这些蛋白是否分泌的了解甚少。在这项研究中，我们表征了主要唾液腺，舌头，牙龈和颊粘膜中HBD-1和HBD-2 mRNA的表达，并检测了唾液分泌物中的β-防御素肽。通过RNase保护试验定量防御素mRNA表达。在牙龈，腮腺，颊粘膜和舌头中检测到HBD-1 mRNA表达。仅在牙龈粘膜中检测到HBD-2 mRNA的表达，并且在具有相关炎症的组织中含量最高。为了测试β-防御素的表达是否可诱导，将牙龈角质形成细胞培养物用白介素-1β（IL-1β）或细菌脂多糖（LPS）处理24小时。 IL-1β处理后HBD-2表达增加约16倍，LPS存在时HBD-2表达增加约5倍。 Western免疫印迹，液相色谱和质谱用于鉴定人唾液中的HBD-1和HBD-2肽。人β-防御素在口腔组织中表达，蛋白质在唾液中分泌。 HBD-1表达是组成型的，而HBD-2表达是由IL-1β和LPS诱导的。人β-防御素可能在抵抗口腔微生物的先天防御中发挥重要作用。

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