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首页> 外文期刊>Infection and immunity >Mycobacterium bovis BCG Vaccine Strains Lack narK2 and narX Induction and Exhibit Altered Phenotypes during Dormancy
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Mycobacterium bovis BCG Vaccine Strains Lack narK2 and narX Induction and Exhibit Altered Phenotypes during Dormancy

机译：牛分枝杆菌卡介苗疫苗株在休眠期间缺乏narK2和narX诱导并表现出改变的表型

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Mycobacterium tuberculosis is the causative agent of tuberculosis, a disease that affects one-third of the world's population. The sole extant vaccine for tuberculosis is the live attenuated Mycobacterium bovis bacillus Calmette-Guerin (BCG). We examined 13 representative BCG strains from around the world to ascertain their ability to express DosR-regulated dormancy antigens. These are known to be recognized by T cells of M. tuberculosis-infected individuals, especially those harboring latent infections. Differences in the expression of these antigens could be valuable for use as diagnostic markers to distinguish BCG vaccination from latent tuberculosis. We determined that all BCG strains were defective for the induction of two dormancy genes: narK2 (Rv1737c) and narX (Rv1736c). NarK2 is known to be necessary for nitrate respiration during anaerobic dormancy. Analysis of the narK2/X promoter region revealed a base substitution mutation in all tested BCG strains and M. bovis in comparison to the M. tuberculosis sequence. We also show that nitrate reduction by BCG strains during dormancy was greatly reduced compared to M. tuberculosis and varied between tested strains. Several dormancy regulon transcriptional differences were also identified among the strains, as well as variation in their growth and survival. These findings demonstrate defects in DosR regulon expression during dormancy and phenotypic variation between commonly used BCG vaccine strains.
机译：结核分枝杆菌是结核病的病原体，是一种影响世界三分之一人口的疾病。唯一现存的结核病疫苗是减毒的活牛分枝杆菌卡麦特·格林（BCG）疫苗。我们检查了来自世界各地的13个代表性BCG菌株，以确定它们表达DosR调节的休眠抗原的能力。已知这些被 M的T细胞识别。结核感染的个体，尤其是那些潜伏感染的个体。这些抗原表达的差异对于用作诊断标记物以区分BCG疫苗接种与潜在结核病可能是有价值的。我们确定所有BCG菌株均不能诱导两个休眠基因： narK2 （Rv1737c）和 narX （Rv1736c）。已知NarK2是厌氧休眠期间硝酸盐呼吸所必需的。对 narK2 / X 启动子区域的分析显示，在所有测试的BCG菌株和 M中均存在碱基取代突变。与 M相比，bovis 。结核序列。我们还显示，与 M相比，BCG菌株在休眠期间的硝酸盐还原作用大大降低。结核，并且在测试菌株之间有所不同。菌株之间还鉴定了几种休眠调节子转录差异，以及它们的生长和存活变异。这些发现表明，在常用的BCG疫苗株之间的休眠和表型变异期间，DosR regulon表达存在缺陷。

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