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首页> 外文期刊>Infection and immunity >Cryptosporidium parvum at Different Developmental Stages Modulates Host Cell Apoptosis In Vitro
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Cryptosporidium parvum at Different Developmental Stages Modulates Host Cell Apoptosis In Vitro

机译：处于不同发育阶段的小隐孢子虫在体外调节宿主细胞凋亡。

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We studied apoptosis in a human ileocecal adenocarcinoma tumor cell line (HCT-8) infected with Cryptosporidium parvum, from 2 to 72 h postinfection (h.p.i.). At 2 h.p.i., the percentage of annexin V-positive cells in the cell culture had increased to 10% compared to 2.5% in noninfected control culture; sorted infected cells expressed mRNA of FasL, the active form of caspase 3, and high caspase 3 activity, whereas the noninfected neighboring cells sorted from the same culture showed no signs of apoptosis. At 24 h.p.i., the percentages of early (annexin V positive) and late (DNA fragment) apoptotic cells were 13 and 2%, respectively, in the entire cell culture, and these percentages were not statistically significant in comparison with those from noninfected control cultures. At this time, sorted infected cells expressed the inactive form of caspase 3, a low caspase 3 activity, and the antiapoptotic protein Bcl-2. Noninfected cells sorted from the same culture showed expression of the active form of caspase 3, a moderate caspase 3 activity, and no Bcl-2 expression. At 48 h.p.i., the percentages of early and late apoptotic cells and caspase 3 activity had increased in the total cell culture, and both sorted infected and noninfected cells showed the active form of caspase 3. These results show that C. parvum, depending on its developmental stage, can inhibit (at the trophozoite stage) or promote (at the sporozoite and merozoite stages) host cell apoptosis, suggesting that it is able to interact with and regulate the host-cell gene expression.
机译：我们在感染后2至72小时（h.p.i.）研究了感染了 Cryptosporidium parvum 的人回盲肠腺癌细胞系（HCT-8）的细胞凋亡。在2 h.p.i.时，细胞培养物中膜联蛋白V阳性细胞的百分比已增加至10％，而未感染的对照培养物中为2.5％;分选的感染细​​胞表达FasL的mRNA，caspase 3的活性形式和高的caspase 3活性，而从同一培养物中分选出的未感染的邻近细胞则没有凋亡迹象。在24 hpi时，整个细胞培养物中早期（annexin V阳性）和晚期（DNA片段）凋亡细胞的百分比分别为13％和2％，与未感染的对照培养相比，这些百分比没有统计学意义。此时，分选的感染细​​胞表达了caspase 3的失活形式，caspase 3活性低和抗凋亡蛋白Bcl-2。从同一培养物中分选出的未感染细胞显示出caspase 3活性形式的表达，中等的caspase 3活性，并且没有Bcl-2表达。在48 h.p.i.，总细胞培养物中早期和晚期凋亡细胞的百分比和caspase 3活性均增加，分选的感染细​​胞和未感染细胞均显示caspase 3的活性形式。这些结果表明 C。幼虫取决于其发育阶段，可以抑制（在滋养体阶段）或促进（在子孢子和裂殖子阶段）宿主细胞凋亡，表明它能够与宿主细胞基因表达相互作用并调节其表达。

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