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首页> 外文期刊>Infection and immunity >Expression of Haemophilus ducreyi Collagen Binding Outer Membrane Protein NcaA Is Required for Virulence in Swine and Human Challenge Models of Chancroid
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Expression of Haemophilus ducreyi Collagen Binding Outer Membrane Protein NcaA Is Required for Virulence in Swine and Human Challenge Models of Chancroid

机译：猪和人类挑战模型的毒力需要嗜血杜克雷胶原蛋白结合外膜蛋白NcaA的表达。

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Haemophilus ducreyi, the etiologic agent of the sexually transmitted genital ulcer disease chancroid, has been shown to associate with dermal collagen fibers within infected skin lesions. Here we describe NcaA, a previously uncharacterized outer membrane protein that is important for H. ducreyi collagen binding and host colonization. An H. ducreyi strain lacking the ncaA gene was impaired in adherence to type I collagen but not fibronectin (plasma or cellular form) or heparin. The mutation had no effect on serum resistance or binding to HaCaT keratinocytes or human foreskin fibroblasts in vitro. Escherichia coli expressing H. ducreyi NcaA bound to type I collagen, demonstrating that NcaA is sufficient to confer collagen attachment. The importance of NcaA in H. ducreyi pathogenesis was assessed using both swine and human experimental models of chancroid. In the swine model, 20% of lesions from sites inoculated with the ncaA mutant were culture positive for H. ducreyi 7 days after inoculation, compared to 73% of wild-type-inoculated sites. The average number of CFU recovered from mutant-inoculated lesions was also significantly reduced compared to that recovered from wild-type-inoculated sites at both 2 and 7 days after inoculation. In the human challenge model, 8 of 30 sites inoculated with wild-type H. ducreyi progressed to the pustular stage, compared to 0 of 30 sites inoculated with the ncaA mutant. Together these results demonstrate that the collagen binding protein NcaA is required for H. ducreyi infection.
机译：性传播生殖器溃疡疾病类c虫的病原体 Duemyi 已被证明与感染的皮肤病灶中的真皮胶原纤维有关。在这里，我们描述了NcaA，这是以前未表征的外膜蛋白，对H非常重要。 ducreyi 胶原结合和宿主定植。一个 H。缺乏 ncaA 基因的ducreyi 菌株对I型胶原的粘附能力受损，但对纤连蛋白（血浆或细胞形式）或肝素的粘附能力受损。在体外，该突变对血清抗性或与HaCaT角质形成细胞或人包皮成纤维细胞的结合没有影响。表达 H的大肠杆菌。 ducreyi NcaA与I型胶原蛋白结合，表明NcaA足以赋予胶原蛋白附着力。 NcaA在 H中的重要性。杜克雷伊的发病机理是通过猪和人的类chan虫实验模型评估的。在猪模型中， ncaA 突变株接种部位的20％病变对 H培养呈阳性。接种后第7天加入ducreyi ，而野生型接种位点占73％。与在接种后第2天和第7天从野生型接种位点回收的CFU相比，从突变型接种病灶回收的CFU的平均数目也显着减少。在人类挑战模型中，野生型H接种了30个站点中的8个。 ducreyi 进入脓疱期，相比之下， ncaA 突变疫苗接种的30个位点中有0个位点。这些结果共同表明， H需要胶原结合蛋白NcaA。 ducreyi 感染。

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