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首页> 外文期刊>Infection and immunity >A DNA Prime-Mycobacterium bovis BCG Boost Vaccination Strategy for Cattle Induces Protection against Bovine Tuberculosis
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A DNA Prime-Mycobacterium bovis BCG Boost Vaccination Strategy for Cattle Induces Protection against Bovine Tuberculosis

机译：DNA牛牛分枝杆菌BCG加强疫苗接种策略可诱导牛结核病防护

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The variable efficacy of bacillus Calmette-Guérin (Mycobacterium bovis BCG) in protecting humans and cattle against tuberculosis has prompted a search for a more effective vaccination regimen. A prime-boost strategy was investigated in cattle naturally sensitized to environmental mycobacteria by using a combination of three DNA vaccines coding for Hsp 65, Hsp 70, and Apa for priming, followed by a boost with BCG prior to experimental challenge with virulent M. bovis. Controls were vaccinated with DNA or BCG alone or were not vaccinated. The immune responses were monitored throughout the study, and protection was assessed based on reductions in the numbers of lesions and viable mycobacteria in lymph node samples. Vaccination with BCG alone or with a DNA prime-BCG boost regimen induced high levels of antigen-specific gamma interferon (IFN-γ) in whole-blood cultures. In the prime-boost group there were fewer animals with severe lung lesions, fewer lymph nodes with lesions per animal, a smaller proportion of animals with lesions, lower mean lung and lymph node lesion scores, and less M. bovis isolated from retropharyngeal and thoracic lymph nodes compared to the results obtained for the nonvaccinated animals. The prime-boost regimen induced significant enhancement of protection in six parameters, compared with significant enhancement of protection in only two parameters for BCG alone. In addition, following challenge, in vitro IFN-γ responses against ESAT-6 and CFP-10, as well as bovine tuberculin-induced skin test and in vitro IFN-γ responses, were identified as immunological markers that predicted protection. The use of the prime-boost strategy suggested that a combination of vaccines may be better than a single vaccine for protection against tuberculosis.
机译：卡介菌（牛分枝杆菌 BCG）在保护人和牛免受结核病方面的可变功效促使人们寻找更有效的疫苗接种方案。通过使用三种编码Hsp 65，Hsp 70和Apa的DNA疫苗的组合，对自然对环境分枝杆菌敏感的牛进行了初免-加强策略研究，然后在受到强毒性的实验性攻击之前先用BCG加强免疫M.对照组只接种了DNA或BCG疫苗或未接种过疫苗。在整个研究过程中都对免疫反应进行了监测，并根据淋巴结样本中病灶数量和存活分枝杆菌数量的减少来评估保护作用。在全血培养中，单独使用BCG或DNA初免-BCG加强疫苗接种会诱导高水平的抗原特异性γ干扰素（IFN-γ）。在初免-升压组中，有严重肺部病变的动物较少，每只动物具有病变的淋巴结较少，有病变的动物比例较小，平均肺和淋巴结病变得分较低，M较低。从咽后和胸腔淋巴结中分离出的牛与未接种疫苗的动物相比。与仅单独使用BCG的仅两个参数的保护作用显着增强相比，初免-升压方案可在六个参数的条件下显着增强保护作用。此外，挑战后，针对ESAT-6和CFP-10的体外IFN-γ反应以及牛结核菌素诱导的皮肤试验和体外IFN-γ反应被确定为可预测保护作用的免疫学标记。初免-加强策略的使用表明，针对肺结核的保护，疫苗组合可能比单一疫苗更好。

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