Effect of Mycobacterium bovis BCG Vaccination on Mycobacterium-Specific Cellular Proliferation and Tumor Necrosis Factor Alpha Production from Distinct Guinea Pig Leukocyte Populations

Todd M. Lasco; Toshiko Yamamoto; Teizo Yoshimura; Shannon Sedberry Allen; Lynne Cassone; David N. McMurray

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Effect of Mycobacterium bovis BCG Vaccination on Mycobacterium-Specific Cellular Proliferation and Tumor Necrosis Factor Alpha Production from Distinct Guinea Pig Leukocyte Populations

机译：牛分枝杆菌卡介苗接种对豚鼠不同白细胞群分枝杆菌特异性细胞增殖和肿瘤坏死因子α产生的影响

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In this study, we focused on three leukocyte-rich guinea pig cell populations, bronchoalveolar lavage (BAL) cells, resident peritoneal cells (PC), and splenocytes (SPC). BAL cells, SPC, and PC were stimulated either with live attenuated Mycobacterium tuberculosis H37Ra or with live or heat-killed virulent M. tuberculosis H37Rv (multiplicity of infection of 1:100). Each cell population was determined to proliferate in response to heat-killed virulent H37Rv, whereas no measurable proliferative response could be detected upon stimulation with live mycobacteria. Additionally, this proliferative capacity (in SPC and PC populations) was significantly enhanced upon prior vaccination with Mycobacterium bovis BCG. Accordingly, in a parallel set of experiments we found a strong positive correlation between production of antigen-specific bioactive tumor necrosis factor alpha (TNF-α) and prior vaccination with BCG. A nonspecific stimulus, lipopolysaccharide, failed to induce this effect on BAL cells, SPC, and PC. These results showed that production of bioactive TNF-α from mycobacterium-stimulated guinea pig cell cultures positively correlates with the vaccination status of the host and with the virulence of the mycobacterial strain.

机译：在这项研究中，我们集中于三个富白细胞的豚鼠细胞群，支气管肺泡灌洗（BAL）细胞，常驻腹膜细胞（PC）和脾细胞（SPC）。 BAL细胞，SPC和PC可用活的减毒结核分枝杆菌H37Ra或活的或热灭活的剧毒M刺激。结核H37Rv（感染复数为1：100）。确定每个细胞群均能对热杀死的毒性H37Rv进行增殖，而在用活的分枝杆菌刺激后，未检测到可测量的增殖反应。此外，预先接种牛分枝杆菌卡介苗后，这种增殖能力（在SPC和PC人群中）显着增强。因此，在一组平行的实验中，我们发现抗原特异性生物活性肿瘤坏死因子α（TNF-α）的产生与先前接种BCG疫苗之间有很强的正相关性。非特异性刺激脂多糖未能诱导对BAL细胞，SPC和PC的这种作用。这些结果表明，由分枝杆菌刺激的豚鼠细胞培养物产生生物活性TNF-α与宿主的疫苗接种状况以及分枝杆菌菌株的毒力正相关。

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《Infection and immunity》 |2003年第12期|共8页
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Todd M. Lasco; Toshiko Yamamoto; Teizo Yoshimura; Shannon Sedberry Allen; Lynne Cassone; David N. McMurray;
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中图分类医学免疫学;
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1. Mycobacterium bovis BCG vaccination modulates TNF-alpha production after pulmonary challenge with virulent Mycobacterium tuberculosis in guinea pigs. [J] . Yamamoto T, Lasco TM, Uchida K, Tuberculosis . 2007,第2期

机译：在豚鼠中，用强力结核分枝杆菌进行肺攻击后，牛分枝杆菌BCG疫苗接种可调节TNF-α的产生。
2. In vitro effects of 1,25-dihydroxyvitamin D3 on interferon-gamma and tumor necrosis factor-alpha secretion by blood leukocytes from young and adult cattle vaccinated with Mycobacterium bovis BCG. [J] . Nonnecke BJ, Waters WR, Foote MR, International Journal for Vitamin and Nutrition Research: Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung . 2003,第4期

机译：1,25-二羟基维生素D3对用牛分枝杆菌BCG接种的成年和成年牛的血液白细胞分泌干扰素-γ和肿瘤坏死因子-α的体外作用。
3. Increased Pulmonary Tumor Necrosis Factor Alpha, Interleukin-6 (IL-6), and IL-17A Responses Compensate for Decreased Gamma Interferon Production in Anti-IL-12 Autovaccine-Treated, Mycobacterium bovis BCG-Vaccinated Mice [J] . Danielle Freches, Marta Romano, Hannelie Korf, Clinical and vaccine immunology: CVI . 2011,第1期

机译：增加的肺肿瘤坏死因子α，白介素6（IL-6）和IL-17A反应补偿抗IL-12自体疫苗治疗的牛分枝杆菌BCG疫苗接种的小鼠中γ干扰素产生的减少。
4. Tumor Necrosis Factor-alpha (TNF-alpha) Gene -238 Single Nucleotide Polymorphism (SNP) Is Associated with Low TNF-a Production from Peripheral Blood Mononuclear Cells (PBMCs) in Japanese [C] . K. Shuno, M. Aoyama, S. Fudo, Conference on Shock . 2008

机译：肿瘤坏死因子-α（TNF-α）基因-238单核苷酸多态性（SNP）与日本外周血单核细胞（PBMC）的低TNF-A生产相关
5. New insights into tumor necrosis factor-alpha in cancer: Distinct isoforms exert opposing effects on tumor associated myeloid cells and tumorigenesis. [D] . Ardestani, Shidrokh. 2013

机译：对癌症中肿瘤坏死因子-α的新见解：不同的同工型对肿瘤相关的髓样细胞和肿瘤发生具有相反的作用。
6. Effect of Mycobacterium bovis BCG Vaccination on Mycobacterium-Specific Cellular Proliferation and Tumor Necrosis Factor Alpha Production from Distinct Guinea Pig Leukocyte Populations [O] . Todd M. Lasco, Toshiko Yamamoto, Teizo Yoshimura, 2003

机译：牛分枝杆菌卡介苗接种对豚鼠不同白细胞群分枝杆菌特异性细胞增殖和肿瘤坏死因子α产生的影响
7. Effect of Mycobacterium bovis BCG Vaccination on Mycobacterium-Specific Cellular Proliferation and Tumor Necrosis Factor Alpha Production from Distinct Guinea Pig Leukocyte Populations [O] . Lasco, Todd M., Yamamoto, Toshiko, Yoshimura, Teizo, 2003

机译：牛分枝杆菌卡介苗接种对豚鼠不同白细胞群分枝杆菌特异性细胞增殖和肿瘤坏死因子α产生的影响
8. Production of Tumor Necrosis Factor-alpha, Interleukin 1-beta, Interleukin 2, and Interleukin 6 by Rat Leukocyte Subpopulations after Exposure to Substance P. [R] . Delgado, A. V., McManus, A. T., Chambers, J. P. 2003

机译：暴露于物质p后大鼠白细胞亚群产生肿瘤坏死因子-α，白细胞介素1-β，白细胞介素2和白细胞介素6

Effect of Mycobacterium bovis BCG Vaccination on Mycobacterium-Specific Cellular Proliferation and Tumor Necrosis Factor Alpha Production from Distinct Guinea Pig Leukocyte Populations

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