Infection with an Avirulent phoP Mutant of Neisseria meningitidis Confers Broad Cross-Reactive Immunity

J. Newcombe; L.-J. Eales-Reynolds; L. Wootton; A. R. Gorringe; S. G. P. Funnell; S. C. Taylor; J. J. McFadden

首页> 外文期刊>Infection and immunity >Infection with an Avirulent phoP Mutant of Neisseria meningitidis Confers Broad Cross-Reactive Immunity

【24h】

Infection with an Avirulent phoP Mutant of Neisseria meningitidis Confers Broad Cross-Reactive Immunity

机译：脑膜炎奈瑟氏球菌无毒phoP突变体的感染具有广泛的交叉反应性免疫力

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Successful vaccines against serogroup A and C meningococcal strains have been developed, but current serogroup B vaccines provide protection against only a limited range of strains. The ideal meningococcal vaccine would provide cross-reactive immunity against the variety of strains that may be encountered in any community, but it is unclear whether the meningococcus possesses immune targets that have the necessary level of cross-reactivity. We have generated a phoP mutant of the meningococcus by allele exchange. PhoP is a component of a two-component regulatory system which in other bacteria is an important regulator of virulence gene expression. Inactivation of the PhoP-PhoQ system in Salmonella leads to avirulence, and phoP mutants have been shown to confer protection against virulent challenge. These mutants have been examined as potential live attenuated vaccines. We here show that a phoP mutant of the meningococcus is avirulent in a mouse model of infection. Moreover, infection of mice with the phoP mutant stimulated a bactericidal immune response that not only killed the infecting strain but also showed cross-reactive bactericidal activity against a range of strains with different serogroup, serotype, and serosubtyping antigens. Sera from the mutant-infected mice contained immunoglobulin G that bound to the surface of a range of meningococcal strains and mediated opsonophagocytosis of meningococci by human phagocytic cells. The meningococcal phoP mutant is thus a candidate live, attenuated vaccine strain and may also be used to identify cross-reactive protective antigens in the meningococcus.

机译：已经开发出针对血清群A和C脑膜炎球菌菌株的成功疫苗，但是目前的血清群B疫苗仅针对有限范围的菌株提供保护。理想的脑膜炎球菌疫苗将提供针对任何社区可能遇到的多种菌株的交叉反应性免疫力，但是尚不清楚脑膜炎球菌是否具有具有必要交叉反应性水平的免疫靶标。我们已经通过等位基因交换产生了脑膜炎球菌的 phoP 突变体。 PhoP是两组分调节系统的组成部分，在其他细菌中，它是毒力基因表达的重要调节剂。沙门氏菌（Salmonella）中PhoP-PhoQ系统的失活会导致无毒力，并且显示 phoP 突变体可提供针对强毒攻击的保护作用。这些突变体已被视为潜在的减毒活疫苗。我们在这里显示脑膜炎球菌的 phoP 突变体在小鼠感染模型中是无毒的。而且，用 phoP 突变体感染的小鼠刺激了杀菌免疫反应，不仅杀死了感染菌株，而且还针对一系列具有不同血清群，血清型和血清分型抗原的菌株表现出交叉反应杀菌活性。。来自突变感染小鼠的血清含有免疫球蛋白G，其与一系列脑膜炎球菌菌株的表面结合，并由人吞噬细胞介导脑膜炎球菌的调理吞噬作用。因此，脑膜炎球菌 phoP 突变体是候选的减毒活疫苗株，也可用于鉴定脑膜炎球菌中的交叉反应性保护性抗原。 展开▼

著录项

来源
《Infection and immunity》 |2004年第1期|共7页

作者
J. Newcombe; L.-J. Eales-Reynolds; L. Wootton; A. R. Gorringe; S. G. P. Funnell; S. C. Taylor; J. J. McFadden;
展开▼

作者单位

展开▼

收录信息

原文格式 PDF

正文语种

中图分类医学免疫学;

关键词

相似文献

外文文献

中文文献

专利

1. Generation and characterization of a PhoP homologue mutant of Neisseria meningitidis. [J] . Johnson CR, Newcombe J, Thorne S, Molecular Microbiology . 2001,第5期

机译：脑膜炎奈瑟氏球菌PhoP同源突变体的产生和表征。

2. Immuno-proteomic analysis of human immune responses to experimental Neisseria meningitidis outer membrane vesicle vaccines identifies potential cross-reactive antigens [J] . Williams Jeannette N, Weynants Vincent, Poolman Jan T, Vaccine . 2014,第11期

机译：人类对实验性脑膜炎奈瑟氏球菌外膜囊泡疫苗的免疫反应的免疫蛋白质组学分析确定了潜在的交叉反应抗原

3. Immuno-proteomic analysis of human immune responses to experimental Neisseria meningitidis outer membrane vesicle vaccines identifies potential cross-reactive antigens [J] . Williams Jeannette N, Weynants Vincent, Poolman Jan T, Vaccine . 2014,第11期

机译：人类对实验性脑膜炎奈瑟氏球菌外膜囊泡疫苗的免疫反应的免疫蛋白质组学分析确定了潜在的交叉反应抗原

4. Top-down mass spectrometry probes immune evasion by pathogenic Neisseria meningitidis [C] . Julia Chamot-Rooke, Joseph Gault, Christian Malosse, International Mass Spectrometry Conference . 2014

机译：自上而下的质谱探针免疫逃避致病性奈良奈瑟

5. REGULATION OF IRON METABOLISM DURING NEISSERIA MENINGITIDIS INFECTION IN MICE. [D] . LETENDRE, ELAINE DANIELE. 1984

机译：小鼠奈瑟氏球菌感染期间铁代谢的调节。

6. Infection with an Avirulent phoP Mutant of Neisseria meningitidis Confers Broad Cross-Reactive Immunity [O] . J. Newcombe, L.-J. Eales-Reynolds, L. Wootton, 2004

机译：脑膜炎奈瑟氏球菌无毒phoP突变体的感染具有广泛的交叉反应性免疫力

7. Infection with an avirulent phoP mutant of Neisseria meningitidis confers broad cross-reactive immunity [O] . Newcombe, J, Eales-Reynolds, L, Wootton, L, 2004

机译：脑膜炎奈瑟氏球菌的无毒phoP突变株感染可产生广泛的交叉反应性免疫

1. 脑膜炎奈瑟氏球菌感染中毒症1例 [J] . 陶思 ,汤多壮 ,汤屹 . 内科急危重症杂志 . 2008,第003期

2. 呼吸道和男性泌尿生殖道非脑膜炎球菌与非淋球菌奈瑟菌感染的实验诊断与临床研究 [J] . 王丹霓 ,王和 ,罗振华 . 贵州医药 . 2009,第003期

3. 2013-2015年北京市门头沟区健康人群脑膜炎奈瑟氏球菌带菌率分析 [J] . 刘海涛 ,庄国良 ,王志越 . 首都公共卫生 . 2016,第002期

4. A群奈瑟氏脑膜炎球菌荚膜多糖-破伤风类毒素结合疫苗的制备及其免疫原性研究 [J] . 刘方蕾 ,吴兵 ,杜琳 . 微生物学免疫学进展 . 2005,第002期

5. C群奈瑟氏脑膜炎球菌荚膜多糖-TT结合疫苗的制备及其在小鼠体内免疫原性的研究 [J] . 吴兵 ,刘方蕾 ,崔萱林 . 微生物学免疫学进展 . 2005,第003期

6. 脑膜炎奈瑟菌感染的研究现况 [C] . 朱启镕 . 第十一届全国儿科感染性疾病学术会议 . 2013

7. 脑膜炎奈瑟球菌外膜蛋白NMB0315的晶体结构研究 [A] . 王向宇 . 2014

1. 含重组菌毛蛋白的抗淋病奈瑟氏球茵或脑膜炎奈瑟氏球菌的疫苗 [P] . 中国专利： CN1245512C . 2006.03.15

2. 含重组菌毛蛋白的抗淋病奈瑟氏球茵或脑膜炎奈瑟氏球菌的疫苗 [P] . 中国专利： CN1298446A . 2001-06-06

3. pencicillin-binding protein, nucleic acid, antibody or antibody fragment, medicament, pharmaceutical composition, uses of at least one penicillin-binding protein or fragment or variant or fragment thereof, of at least one nucleic acid and of at least one antibody or antibody fragment, in vitro antibody detection methods directed against neisseria meningitidis infection from a penicillin-binding protein, nucleic acid, antibody or an antibody fragment, pharmaceutical composition of at least one penicillin-binding protein or fragment or variant or variant thereof, of at least one nucleic acid and at least one antibody directed against neisseria meningitidis infection from a biological sample of a mammal and in vitro diagnosis of neisseria miningitidis infection from a biological sample from a mammal and monoclonal antibody [P] . 外国专利： BRPI0610802A2 . 2016-11-16

机译：青霉素结合蛋白，核酸，抗体或抗体片段，药物，药物组合物，至少一种青霉素结合蛋白或其片段或变异体或片段，至少一种核酸和至少一种抗体或抗体片段的用途，针对脑膜炎奈瑟菌的青霉素结合蛋白，核酸，抗体或抗体片段感染的体外抗体检测方法，至少一种青霉素结合蛋白或其片段或变异体或变异体，至少一种核酸的药物组合物酸和至少一种针对哺乳动物生物样品中脑膜炎奈瑟氏菌感染的抗体以及来自哺乳动物生物样品中的矿业性奈瑟氏球菌感染的体外诊断和单克隆抗体

4. COMPOSITIONS OF VACCINES FOR TREATMENT OR PREVENTION OF INFECTION OR DISEASE, CAUSED BY Neisseria meningitidis AND/OR Neisseria gonorrhoeae, CONTAINING MUTANT PROTEIN, BINDING H FACTOR [P] . 外国专利： RU2559528C2 . 2015-08-10

机译：脑膜炎奈瑟氏球菌和/或淋病奈瑟氏球菌引起的用于治疗或预防感染或疾病的疫苗的成分，含有突变蛋白，结合了H因子

5. Method of preparation of crystals of infections, and their applications for the detection and the prevention of infections due to neisseria gonorrhoeae neisseria meningitidis non-species-related breakpoints * [P] . 外国专利： FR2308377B1 . 1979-04-06

机译：感染晶体的制备方法及其在淋病奈瑟氏球菌脑膜炎奈瑟氏球菌非物种相关断点的检测和预防中的应用*

相关主题

Infection with an Avirulent phoP Mutant of Neisseria meningitidis Confers Broad Cross-Reactive Immunity

摘要

著录项

相似文献

相关主题

期刊订阅