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首页> 外文期刊>Infection and immunity >Capsule Expression and Genotypic Differences among Staphylococcus aureus Isolates from Patients with Chronic or Acute Osteomyelitis
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Capsule Expression and Genotypic Differences among Staphylococcus aureus Isolates from Patients with Chronic or Acute Osteomyelitis

机译：慢性或急性骨髓炎患者金黄色葡萄球菌分离物的胶囊表达和基因型差异

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There is ample evidence that Staphylococcus aureus capsular polysaccharide (CP) promotes virulence. Loss of capsule expression, however, may lead to S. aureus persistence in a chronically infected host. This study was conducted to determine the relative prevalence of nonencapsulated S. aureus in patients with chronic and acute osteomyelitis. Only 76/118 (64%) S. aureus isolates from patients with osteomyelitis expressed CP, whereas all 50 isolates from blood cultures of patients with infections other than osteoarticular infections expressed CP (P = 0.0001). A significantly higher prevalence of nonencapsulated S. aureus was found in patients with chronic osteomyelitis (53%) than in those with acute osteomyelitis (21%) (P = 0.0046). S. aureus isolates obtained from multiple specimens from five of six patients with chronic osteomyelitis exhibited phenotypic (expression of CP, α-hemolysin, β-hemolysin, slime, and the small-colony variant phenotype) and/or genotypic (pulsed-field gel electrophoresis and spa typing) differences. Nonencapsulated S. aureus was recovered from at least one specimen from each chronic osteomyelitis patient. Fourteen isolates obtained from two patients with acute osteomyelitis were indistinguishable from each other within each group, and all produced CP5. In conclusion, we demonstrated that nonencapsulated S. aureus is more frequently isolated from patients with chronic osteomyelitis than from those with acute osteomyelitis, suggesting that loss of CP expression may be advantageous to S. aureus during chronic infection. Our findings on multiple S. aureus isolates from individual patients allow us to suggest that selection of nonencapsulated S. aureus is likely to have occurred in the patient during long-term bone infection.
机译：有充分的证据表明金黄色葡萄球菌荚膜多糖（CP）可以提高毒力。然而，胶囊表达的丧失可能导致 S。长期感染宿主中的金黄色葡萄球菌持续存在。进行这项研究以确定未封装的 S的相对患病率。慢性和急性骨髓炎患者的金黄色葡萄球菌。只有76/118（64％） S。骨髓炎患者的金黄色葡萄球菌分离株表达CP，而除骨关节感染以外的其他感染者血液培养的50株分离株表达CP（ P = 0.0001）。未封装的 S的患病率明显更高。慢性骨髓炎患者中发现金黄色葡萄球菌（53％），而急性骨髓炎患者中发现金黄色葡萄球菌（21％）（ P = 0.0046）。 S。从六名慢性骨髓炎患者中五名患者的多个标本中分离出的金黄色葡萄球菌表现出表型（CP，α-溶血素，β-溶血素，粘液和小菌落变异表型的表达）和/或基因型（脉冲-场凝胶电泳和 spa 类型）的差异。未封装的 S。从每位慢性骨髓炎患者的至少一个标本中回收金黄色葡萄球菌。在两组中，从两名患有急性骨髓炎的患者中分离出的十四株分离株彼此无区别，并且全部产生了CP5。总之，我们证明了未封装的 S。慢性骨髓炎患者比急性骨髓炎患者更常分离出金黄色葡萄球菌，这表明CP表达的丧失可能对 S有利。慢性感染中的金黄色。我们在多个 S上的发现。从个体患者中分离出的金黄色葡萄球菌使我们建议选择非包囊的 S。长期的骨感染患者中很可能会发生金黄色葡萄球菌。

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