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首页> 外文期刊>Infection and immunity >Circulating γδ T Cells in Response to Salmonella enterica Serovar Enteritidis Exposure in Chickens
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Circulating γδ T Cells in Response to Salmonella enterica Serovar Enteritidis Exposure in Chickens

机译：鸡肠道沙门氏菌血清肠炎沙门氏菌暴露对循环γδT细胞的响应

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γδT cells are considered crucial to the outcome of various infectious diseases. The present study was undertaken to characterizeγδ (T-cell receptor 1+ [TCR1+]) T cells phenotypically and functionally in avian immune response. Day-old chicks were orally immunized with Salmonella enterica serovar Enteritidis live vaccine or S. enterica serovar Enteritidis wild-type strain and infected using the S. enterica serovar Enteritidis wild-type strain on day 44 of life. Between days 3 and 71, peripheral blood was examined flow cytometrically for the occurrence of γδ T-cell subpopulations differentiated by the expression of T-cell antigens. Three different TCR1+ cell populations were found to display considerable variation regarding CD8α antigen expression: (i) CD8α+high TCR1+ cells, (ii) CD8α+dim TCR1+ cells, and (iii) CD8α? TCR1+ cells. While most of the CD8α+high TCR1+ cells expressed the CD8αβ heterodimeric antigen, the majority of the CD8α+dim TCR1+ cells were found to express the CD8αα homodimeric form. After immunization, a significant increase of CD8αα+high γδ T cells was observed within the CD8α+high TCR1+ cell population. Quantitative reverse transcription-PCR revealed reduced interleukin-7 receptor α (IL-7Rα) and Bcl-x expression and elevated IL-2Rα mRNA expression of the CD8αα+highγδ T cells. Immunohistochemical analysis demonstrated a significant increase of CD8α+ and TCR1+ cells in the cecum and spleen and a decreased percentage of CD8β+ T cells in the spleen after Salmonella immunization. After infection of immunized animals, immune reactions were restricted to intestinal tissue. The study showed that Salmonella immunization of very young chicks is accompanied by an increase of CD8αα+high γδ T cells in peripheral blood, which are probably activated, and thus represent an important factor for the development of a protective immune response to Salmonella organisms in chickens.
机译：γδT细胞被认为对各种传染病的后果至关重要。本研究旨在鉴定γδ（T细胞受体1 + [TCR1 + ]）T细胞在禽类免疫应答中的表型和功能。用沙门氏菌血清肠炎沙门氏菌活疫苗或 S口服免疫日龄雏鸡。肠炎肠炎血清型肠炎沙门氏菌野生型菌株，并用 S感染。生命第44天时出现的肠炎血清型肠炎肠炎野生型。在第3天到第71天之间，用流式细胞仪检查外周血中是否存在由T细胞抗原表达所区分的γδT细胞亚群。发现三种不同的TCR1 + 细胞群体在CD8α抗原表达方面表现出可观的变异：（i）CD8α + high TCR1 + 细胞，（ii ）CD8α + dim TCR1 + 细胞，以及（iii）CD8α？ TCR1 + 细胞。尽管大多数CD8α + high TCR1 + 细胞表达CD8αβ异二聚体抗原，但大多数CD8α + dim TCR1 + <发现细胞表达CD8αα同二聚体形式。免疫后，CD8α + high TCR1 + 细胞群中CD8αα + high γδT细胞明显增加。定量逆转录-PCR显示CD8αα +高γδT细胞的白介素7受体α（IL-7Rα）和Bcl-x表达降低，IL-2RαmRNA表达升高。免疫组织化学分析显示，盲肠和脾脏中CD8α + 和TCR1 + 细胞显着增加，而CD8β + T细胞百分比降低。沙门氏菌免疫后的脾脏。感染免疫动物后，免疫反应仅限于肠道组织。研究表明，沙门氏菌免疫非常年轻的雏鸡会伴随外周血CD8αα + high γδT细胞的增加，这很可能被激活，因此代表了重要的鸡对沙门氏菌的保护性免疫应答发生的机制。

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