• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Biogenesis of Leishmania major-Harboring Vacuoles in Murine Dendritic Cells
【24h】

Biogenesis of Leishmania major-Harboring Vacuoles in Murine Dendritic Cells

机译：鼠树突状细胞中利什曼原虫主要携带液泡的生物发生。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In mammalian hosts, Leishmania sp. parasites are obligatory intracellular organisms that invade macrophages and dendritic cells (DC), where they reside in endocytic organelles termed parasitophorous vacuoles (PV). Most of the present knowledge of the characteristics of PV harboring Leishmania sp. is derived from studies with infected macrophages. Since DC play a key role in host resistance to leishmaniasis, there is a need to understand the properties and biogenesis of PV in Leishmania sp.-infected DC. Therefore, we determined the acquisition of endosomal and lysosomal molecules by Leishmania major-containing compartments in DC at different maturation stages, using fluorescence labeling and confocal microscopy. The results show that newly formed phagosomes in DC rapidly develop into late endosomal compartments. However, the small GTPase Rab7, which regulates late fusion processes, was found only in PV of mature bone marrow-derived DC (BMDC); it was absent in immature BMDC, suggesting an arrest of their PV biogenesis at the stage of late endosomes. Indeed, fusion assays with endocytic tracers demonstrated that the fusion activity of L. major-harboring PV toward lysosomes is higher in mature BMDC than in immature BMDC. The inhibition of PV-lysosome fusion in DC is dependent upon the viability and life cycle stage of the parasite, because live promastigotes blocked the fusion almost completely, whereas killed organisms and amastigotes induced a considerable level of fusion activity. The differences in the fusion competences of immature and mature DC may be relevant for their distinct functional activities in the uptake, transport, and presentation of parasite antigens.
机译：在哺乳动物宿主中，利什曼原虫 sp.。寄生虫是侵入巨噬细胞和树突状细胞（DC）的强制性细胞内生物，它们位于称为寄生虫空泡（PV）的内吞细胞器中。目前，大多数有关 Leishmania sp的PV特性的知识。来源于感染巨噬细胞的研究。由于DC在宿主对利什曼病的抵抗中起关键作用，因此有必要了解 Leishmania sp。感染的DC中PV的性质和生物发生。因此，我们使用荧光标记和共聚焦显微镜，确定了在成熟期不同阶段，由DC中含有 Leishmania major 的小室获取的内体和溶酶体分子。结果表明，DC中新形成的吞噬体迅速发育成晚期的内体区室。然而，仅在成熟的骨髓源性DC（BMDC）的PV中发现了调控晚融合过程的小GTPase Rab7。在未成熟的BMDC中不存在这种现象，这表明它们的PV生物发生在晚期内体阶段被阻止。确实，用胞吞示踪剂进行融合测定证明了 L的融合活性。成熟的BMDC中，对溶酶体的主要的PV值高于未成熟的BMDC。 DC中PV-溶酶体融合的抑制取决于寄生虫的生存力和生命周期阶段，因为活前鞭毛体几乎完全阻断了融合，而被杀死的生物和变形虫诱导了相当水平的融合活性。未成熟和成熟DC融合能力的差异可能与其在寄生虫抗原的摄取，转运和呈递中的独特功能活性有关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号