• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Micropatterned Macrophage Analysis Reveals Global Cytoskeleton Constraints Induced by Bacillus anthracis Edema Toxin
【24h】

Micropatterned Macrophage Analysis Reveals Global Cytoskeleton Constraints Induced by Bacillus anthracis Edema Toxin

机译：Micropatterned巨噬细胞分析揭示炭疽芽孢杆菌浮肿毒素诱导的总体细胞骨架约束。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bacillus anthracis secretes the edema toxin (ET) that disrupts the cellular physiology of endothelial and immune cells, ultimately affecting the adherens junction integrity of blood vessels that in turn leads to edema. The effects of ET on the cytoskeleton, which is critical in cell physiology, have not been described thus far on macrophages. In this study, we have developed different adhesive micropatterned surfaces (L and crossbow) to control the shape of bone marrow-derived macrophages (BMDMs) and primary peritoneal macrophages. We found that macrophage F-actin cytoskeleton adopts a specific polar organization slightly different from classical human HeLa cells on the micropatterns. Moreover, ET induced a major quantitative reorganization of F-actin within 16 h with a collapse at the nonadhesive side of BMDMs along the nucleus. There was an increase in size and deformation into a kidney-like shape, followed by a decrease in size that correlates with a global cellular collapse. The collapse of F-actin was correlated with a release of focal adhesion on the patterns and decreased cell size. Finally, the cell nucleus was affected by actin reorganization. By using this technology, we could describe many previously unknown macrophage cellular dysfunctions induced by ET. This novel tool could be used to analyze more broadly the effects of toxins and other virulence factors that target the cytoskeleton.
机译：炭疽芽孢杆菌分泌水肿毒素（ET），该毒素破坏内皮细胞和免疫细胞的细胞生理，最终影响血管的粘附连接完整性，进而导致水肿。迄今为止，尚未对巨噬细胞描述ET对细胞骨架的影响，而ET对细胞生理至关重要。在这项研究中，我们已经开发了不同的胶粘剂微图案表面（L和cross）来控制骨髓巨噬细胞（BMDMs）和原发性腹膜巨噬细胞的形状。我们发现巨噬细胞F-肌动蛋白细胞骨架采用特定的极性组织，其与微模式上的经典人HeLa细胞略有不同。此外，ET诱导F-肌动蛋白在16小时内发生重大的定量重组，并沿细胞核在BMDMs的非黏附侧塌陷。大小增加并变形为肾样形状，然后大小减小，这与整体细胞塌陷有关。 F-肌动蛋白的崩溃与模式上粘着斑的释放和细胞大小的减少有关。最后，细胞核受到肌动蛋白重组的影响。通过使用这项技术，我们可以描述由ET诱导的许多以前未知的巨噬细胞功能障碍。这种新颖的工具可用于更广泛地分析针对细胞骨架的毒素和其他毒力因子的影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号