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首页> 外文期刊>Infection and immunity >Incorporation of Phosphorylcholine into the Lipooligosaccharide of Nontypeable Haemophilus influenzae Does Not Correlate with the Level of Biofilm Formation In Vitro
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Incorporation of Phosphorylcholine into the Lipooligosaccharide of Nontypeable Haemophilus influenzae Does Not Correlate with the Level of Biofilm Formation In Vitro

机译：磷酸胆碱掺入不可分型流感嗜血杆菌的脂寡糖与体外生物膜形成水平无关。

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Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen that causes otitis media in children and community-acquired pneumonia or exacerbations of chronic obstructive pulmonary disease in adults. A large variety of studies suggest that biofilm formation by NTHi may be an important step in the pathogenesis of this bacterium. The objective of this report was to determine the relationship between the presence of phosphorylcholine in the lipooligosaccharide of NTHi and the level of biofilm formation. The study was performed on 111 NTHi clinical isolates collected from oropharyngeal samples of healthy children, middle ear fluid of children with otitis media, and sputum samples of patients with chronic obstructive pulmonary disease or community-acquired pneumonia. NTHi clinical isolates presented a large variation in the level of biofilm formation in a static assay and phosphorylcholine content. Isolates collected from the oropharynx and middle ear fluid of children tended to have more phosphorylcholine and made denser biofilms than isolates collected from sputum samples of patients with chronic obstructive pulmonary disease or community-acquired pneumonia. No correlation was observed between biofilm formation and the presence of phosphorylcholine in the lipooligosaccharide for either planktonic or biofilm growth. This lack of correlation was confirmed by abrogating phosphorylcholine incorporation into lipooligosaccharide through licA gene deletion, which had strain-specific effects on biofilm formation. Altogether, we present strong evidence to conclude that there is no correlation between biofilm formation in a static assay and the presence of phosphorylcholine in lipooligosaccharide in a large collection of clinical NTHi isolates collected from different groups of patients.
机译：非分型流感嗜血杆菌（NTHi）是一种机会病原体，可引起儿童中耳炎和成人社区获得性肺炎或慢性阻塞性肺疾病恶化。大量研究表明，NTHi形成生物膜可能是该细菌发病机理中的重要一步。本报告的目的是确定NTHi脂寡糖中磷酸胆碱的存在与生物膜形成水平之间的关系。该研究是从健康儿童的口咽样本，中耳炎儿童的中耳液以及慢性阻塞性肺疾病或社区获得性肺炎患者的痰液样本中收集的111种NTHi临床分离株进行的。 NTHi临床分离株在静态测定中的生物膜形成水平和磷酸胆碱含量方面存在较大差异。与从慢性阻塞性肺疾病或社区获得性肺炎患者的痰液样本中分离出的分离物相比，从儿童口咽和中耳液中分离出的分离物倾向于具有更多的磷酸胆碱和更厚的生物膜。对于浮游生物膜或生物膜生长，在生物膜形成和脂寡糖中磷酸胆碱的存在之间未观察到相关性。通过将 licA 基因缺失取消了磷酸胆碱掺入脂寡糖中，证实了这种缺乏相关性，这对生物膜的形成具有特定的影响。总而言之，我们提供了强有力的证据来得出结论，即在静态测定中生物膜形成与从不同患者组收集的大量临床NTHi分离物中脂寡糖中磷酸胆碱的存在之间没有关联。

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