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首页> 外文期刊>International Journal of Molecular Sciences >A Set of Novel Microsatellite Markers Developed for a Distylous Species Luculia gratissima (Rubiaceae)
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A Set of Novel Microsatellite Markers Developed for a Distylous Species Luculia gratissima (Rubiaceae)

机译：为a属物种Luculia gratissima（Rubiaceae）开发的一组新型微卫星标记。

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摘要

Luculia gratissima (Wall.) Sweet (Rubiaceae) is a perennial shrub distributed in the southeast margin of the Tibetan Plateau in southwest China and adjacent region of Nepal and Myanmar. The plant is a distylous species with reciprocally placed stigmas and anthers in each floral morph. By using the Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of Sequences Containing (FIASCO) repeats protocol, 19 primer sets were identified in two wild populations. Of these primers, 10 displayed polymorphisms and nine were monomorphic. The number of alleles per locus ranged from two to five, values for observed and expected heterozygosities ranged from 0.000 to 1.000 and from 0.289 to 0.760, with averages of 0.303 and 0.555, respectively. These microsatellite loci will facilitate further studies on breeding system, gene flow patterns, and population structure of L. gratissima and its allied species.
机译：甜菊（Luculia gratissima）（墙）是多年生灌木，分布在中国西南部青藏高原的东南边缘以及尼泊尔和缅甸的邻近地区。该植物是一种离奇种，在每个花形中互有柱头和花药。通过使用包含片段（FIASCO）重复序列的扩增片段长度多态性（AFLP）快速分离技术，在两个野生种群中鉴定出19个引物对。在这些引物中，有10个表现出多态性，而9个是单态性。每个基因座的等位基因数量范围为2至5，观察到的和预期的杂合度值为0.000至1.000和0.289至0.760，平均分别为0.303和0.555。这些微卫星基因座将有助于进一步研究禾本科乳酸杆菌及其相关物种的繁殖系统，基因流动模式和种群结构。

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