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首页> 外文期刊>International Journal of Molecular Sciences >Changes of the Cytoplasmic Proteome in Response to Alcoholic Hepatotoxicity in Rats
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Changes of the Cytoplasmic Proteome in Response to Alcoholic Hepatotoxicity in Rats

机译：大鼠酒精性肝毒性反应中细胞质蛋白质组的变化

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Proteomic analyses have already been used in a number of hepatological studies and provide important information. However, few reports have focused on changes in the cytoplasmic proteome. The present study therefore aimed to evaluate changes in cytoplasmic proteome of rats in response to alcoholic hepatotoxicity. Rats were fed a Liber-DeCarli liquid diet containing ethanol for four weeks. Cytoplasmic proteins except mitochondrial proteins from the livers of these animals were investigated using two-dimensional gel electrophoresis and mass spectrometry. Alcohol induced a decrease in body weight gain and an increase in alanine transaminase (ALT), cholesterol, and phospholipid levels. Histopathological observations revealed hepatic damage characterized by necrosis and fatty change in alcohol-treated group at week 2, which continues until week 4. Our proteomic analysis revealed that 25 proteins were differentially expressed in the ethanol-fed group. Of these, 12 cytoplasmic proteins are being reported for the first time. Taken together, our results provide further insights into the disease mechanism and therapeutic information of alcoholic liver disease.
机译：蛋白质组学分析已用于许多肝病学研究中，并提供重要信息。但是，很少有报道关注细胞质蛋白质组的变化。因此，本研究旨在评估对酒精性肝毒性反应的大鼠细胞质蛋白质组的变化。给大鼠喂食含有乙醇的Liber-DeCarli流质饮食，持续四个星期。使用二维凝胶电泳和质谱法研究了这些动物肝脏中除线粒体蛋白以外的细胞质蛋白。酒精会导致体重增加减少，丙氨酸转氨酶（ALT），胆固醇和磷脂水平增加。组织病理学观察显示，酒精治疗组第2周的肝脏损害以坏死和脂肪变化为特征，这一现象一直持续到第4周。我们的蛋白质组学分析表明，乙醇喂养的组中25种蛋白质差异表达。其中，首次报道了12种胞质蛋白。综上所述，我们的结果为酒精性肝病的发病机理和治疗信息提供了进一步的见解。

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