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首页> 外文期刊>Molecular and Cellular Biology >Characterization of productive and sterile transcripts from the immunoglobulin heavy-chain locus: processing of micron and muS mRNA.
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Characterization of productive and sterile transcripts from the immunoglobulin heavy-chain locus: processing of micron and muS mRNA.

机译：免疫球蛋白重链基因座的生产性和无菌转录本的表征：微米和muS mRNA的加工。

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An analysis of the sizes and sequence content of nuclear RNA transcripts of the heavy-chain locus in two B-cell lymphomas, 70Z/3 and 38C-13, and in selected hybridoma derivatives of 38C has led to the identification of two distinct precursors of the mRNAs encoding the membrane and secretory forms of mu chain. These precursors, termed Pm1 and Ps1, extend from a common 5' terminus (presumably the cap site) to alternative polyadenylation sites located 3' of the membrane and secretory tailpieces, Pm1 and Ps1 are present in similar amounts in lymphomas, indicating roughly equivalent usage of the two polyadenylation sites, whereas Ps1 much greater than Pm1 in hybridomas, indicating that mature plasma cells produce a trans-acting factor which enhances cleavage at the proximal (muS) site. The lymphomas also synthesize several nonproductive or sterile mu (Smu) transcripts from the second H allele. One class of sterile mu transcripts appears to be initiated about 1 kilobase downstream from the JH4 element. In 70Z, in which the nonproductive H allele has undergone a D1J2 fusion, another initiation site was located about 0.3 kilobase upstream of the D1 element. The sterile mu transcripts exhibit the same regulated termination at alternative polyadenylation sites as the mu mRNA precursors, although their rate of production is not necessarily coupled to that of the productive allele. This analysis has also defined probable processing pathways for productive and sterile components in which there is a 5' leads to 3' order for the excision of the large introns.
机译：对两种B细胞淋巴瘤70Z / 3和38C-13以及所选的38C杂交瘤衍生物中重链基因座核RNA转录本大小和序列含量的分析已导致鉴定出两种不同的前体编码μ链膜和分泌形式的mRNA。这些称为Pm1和Ps1的前体，从一个共同的5'端（大概是帽位）延伸到位于膜和分泌尾端3'的另一聚腺苷酸化位点，Pm1和Ps1在淋巴瘤中的存在量相似，表明用法大致相同这两个聚腺苷酸化位点中的Ps1比杂交瘤中的Pm1大得多，这表明成熟的浆细胞会产生反式作用因子，从而增强近端（muS）位点的裂解。淋巴瘤还从第二个H等位基因合成了几个非生产性或无菌mu（Smu）转录本。一类不育的mu转录物似乎在JH4元件下游约1千碱基处开始。在非生产性H等位基因经历了D1J2融合的70Z中，另一个起始位点位于D1元件上游约0.3公里处。无菌的mu转录物在替代的聚腺苷酸化位点显示出与mu mRNA前体相同的调节末端，尽管它们的产生率不一定与生产等位基因的产生率相关。该分析还为生产性和无菌性成分定义了可能的加工途径，其中大内含子的切除存在5'导致3'顺序。

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