Isolation of the amplified dihydrofolate reductase domain from methotrexate-resistant Chinese hamster ovary cells.

J E Looney; J L Hamlin

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Isolation of the amplified dihydrofolate reductase domain from methotrexate-resistant Chinese hamster ovary cells.

机译：从耐甲氨蝶呤的中国仓鼠卵巢细胞中分离扩增的二氢叶酸还原酶结构域。

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We isolated overlapping recombinant cosmids that represent the equivalent of two complete dihydrofolate reductase amplicon types from the methotrexate-resistant CHO cell line CHOC400. The type I amplicons are 260 kilobases long, are arranged in head-to-tail fashion, and represent 10 to 15% of the amplicons in the CHOC400 genome. The type II amplicons are 220 kilobases long, are arranged in head-to-head and tail-to-tail configurations, and constituted the majority of the remaining amplicons in CHOC400 cells. The type II amplicon sequences are represented entirely within the type I unit. These are the first complete amplicons to be cloned from a mammalian cell line.

机译：我们从耐甲氨蝶呤的CHO细胞系CHOC400中分离了代表两种完全相同的二氢叶酸还原酶扩增子类型的重叠重组粘粒。 I型扩增子长260千碱基，以首尾相连的方式排列，代表CHOC400基因组中扩增子的10％至15％。 II型扩增子长220千碱基，头对尾，尾对尾排列，并构成CHOC400细胞中其余大多数扩增子。 II型扩增子序列完全在I型单位内表示。这些是要从哺乳动物细胞系克隆的第一个完整的扩增子。

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《Molecular and Cellular Biology》 |1987年第2期|共9页
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J E Looney; J L Hamlin;
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中图分类细胞生物学;
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1. Overproduction of dihydrofolate reductase and gene amplification in methotrexate-resistant Chinese hamster ovary cells. [J] . W F Flintoff, M K Weber, C R Nagainis, Molecular and Cellular Biology . 1982,第3期

机译：抗甲氨蝶呤的中国仓鼠卵巢细胞中二氢叶酸还原酶的过量生产和基因扩增。
2. Matrix attachment regions are positioned near replication initiation sites, genes, and an interamplicon junction in the amplified dihydrofolate reductase domain of Chinese hamster ovary cells. [J] . P A Dijkwel, J L Hamlin Molecular and Cellular Biology . 1988,第12期

机译：基质附着区域位于中国仓鼠卵巢细胞的扩增的二氢叶酸还原酶结构域中的复制起始位点，基因和两性间连接附近。
3. Short hairpin RNA targeted to dihydrofolate reductase enhances the immunoglobulin G expression in gene-amplified stable Chinese hamster ovary cells. [J] . Wu SC, Hong WW, Liu JH Vaccine . 2008,第38期

机译：靶向二氢叶酸还原酶的短发夹RNA增强了基因扩增的稳定中国仓鼠卵巢细胞中免疫球蛋白G的表达。
4. DEVELOPMENT OF APOPTOSIS-RESISTANT DIHYDROFOLATE REDUCTASE-DEFICIENT CHINESE HAMSTER OVARY CELL LINE [C] . SUK KYOO LEE, GYUN MIN LEE Annual Meeting of the Japanese Association for Animal Cell Technology . 2003

机译：抗凋亡抗性二氢酚酸还原酶缺乏中国仓鼠卵巢细胞系的研制
5. Isolation of a humancDNA for threonyl-tRNA synthetase: Amplification and regulation of the structural gene in Chinese hamster ovary cells. [D] . Kontis, Kris John. 1989

机译：苏糖基-tRNA合成酶的人类cDNA的分离：中国仓鼠卵巢细胞结构基因的扩增和调控。
6. Isolation of the amplified dihydrofolate reductase domain from methotrexate-resistant Chinese hamster ovary cells. [O] . J E Looney, J L Hamlin 1987

机译：从耐甲氨蝶呤的中国仓鼠卵巢细胞中分离扩增的二氢叶酸还原酶结构域。
7. Isolation of the amplified dihydrofolate reductase domain from methotrexate-resistant Chinese hamster ovary cells. [O] . Looney, J E, Hamlin, J L 1987

机译：从耐甲氨蝶呤的中国仓鼠卵巢细胞中分离扩增的二氢叶酸还原酶结构域。
8. In Vitro Chromosome Aberration Test in Chinese Hamster Ovary (CHO-WBL) Cells. [R] . 2016

机译：中国仓鼠卵巢（CHO-WBL）细胞的体外染色体畸变试验。

Isolation of the amplified dihydrofolate reductase domain from methotrexate-resistant Chinese hamster ovary cells.

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