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首页> 外文期刊>Molecular and Cellular Biology >Transcription termination at the chicken beta H-globin gene.
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Transcription termination at the chicken beta H-globin gene.

机译：鸡βH-球蛋白基因的转录终止。

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We characterized the transcription termination region of the chicken beta H-globin gene. First we located the region by nuclear runon transcription in vitro. Then we sequenced and subcloned it into a chloramphenicol acetyltransferase (CAT) expression vector for assay in vivo. The region of beta H termination contains two interesting elements located about 1 kilobase downstream of the beta H gene poly(A) site. Either element alone can block CAT expression if inserted between the promoter and the poly(A) site of the cat gene in pRSVcat. The first element in the termination region is an unusually large inverted repeat in the DNA (delta G = -71 kcal). The second element, 200 base pairs further downstream, is an RNA polymerase II promoter which directs transcription back upstream on the complementary strand. This transcription converges on and collides with that from the beta H gene at or near the inverted repeat where transcription from both directions stops. We propose that the inverted repeat is a strong pause site which positions the converging polymerases for mutual site-specific termination.
机译：我们表征了鸡βH-球蛋白基因的转录终止区。首先，我们通过核子在体外转录定位该区域。然后，我们对其进行测序并将其亚克隆到氯霉素乙酰转移酶（CAT）表达载体中，以进行体内分析。 βH终止区域包含两个有趣的元素，位于βH基因poly（A）位点下游约1千碱基处。如果插入pRSVcat中cat基因的启动子和poly（A）位点之间，则任何一个元素都可以阻止CAT表达。终止区中的第一个元件是DNA中异常大的反向重复序列（δG = -71 kcal）。第二个元件，更下游的200个碱基对，是RNA聚合酶II启动子，可将转录引导回互补链的上游。该转录在反向重复中或附近的βH基因的聚合处会聚，并与之发生冲突，在反向重复处，双向转录均停止。我们提出，反向重复序列是一个强停顿位点，它将聚合聚合酶定位为相互的位点特异性终止。

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