• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Burkitt lymphoma cell line carrying a variant translocation creates new DNA at the breakpoint and violates the hierarchy of immunoglobulin gene rearrangement.
【24h】

Burkitt lymphoma cell line carrying a variant translocation creates new DNA at the breakpoint and violates the hierarchy of immunoglobulin gene rearrangement.

机译：携带变异易位的伯基特淋巴瘤细胞系在断点处产生新的DNA，并违反了免疫​​球蛋白基因重排的层次结构。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Burkitt lymphoma cell line KK124, which contains a reciprocal t(8;22) translocation, was shown to have rearranged in a region 3' to the c-myc proto-oncogene on chromosome 8 and 5' to the lambda constant region on chromosome 22. The breakpoint was cloned and sequenced, revealing that c-myc and a portion of its 3' region abutted a complete lambda variable gene that had undergone V-J recombination. Since this cell line expresses kappa light chain, this lambda rearrangement violates the previously proposed hierarchy of immunoglobulin gene rearrangement. A novel duplication of normal chromosome 8 sequences was also found at the breakpoint. The first exon of c-myc and its flanking sequence from the translocated allele was sequenced and compared with a normal counterpart. Extensive mutation was found within the first exon in contrast to its 3' and 5' flanking regions. S1 nuclease analysis revealed that it was the translocated c-myc being expressed and that there was a promoter shift from P2 to P1. The detailed structural analysis of this cell line provides clues concerning mechanisms of chromosomal translocation and c-myc deregulation in Burkitt lymphomas.
机译：Burkitt淋巴瘤细胞系KK124包含相互的t（8; 22）易位，已显示在8号染色体上c-myc原癌基因的3'区域和5号染色体上的lambda恒定区的区域进行了重排。 22.克隆了断点并进行了测序，揭示了c-myc及其3'区的一部分邻接了经过VJ重组的完整lambda可变基因。由于该细胞系表达κ轻链，因此该λ重排违反了先前提出的免疫球蛋白基因重排的层次结构。在断点处也发现了正常8号染色体序列的新型复制。对c-myc的第一个外显子及其来自易位等位基因的侧翼序列进行了测序，并与正常对应物进行了比较。与第一个外显子的3'和5'侧翼区域相反，发现了广泛的突变。 S1核酸酶分析显示它是被表达的易位c-myc，并且存在从P2到P1的启动子转移。该细胞系的详细结构分析提供了有关Burkitt淋巴瘤中染色体易位和c-myc失调机制的线索。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号