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首页> 外文期刊>Molecular and Cellular Biology >Site-specific mutagenesis of cdc2+, a cell cycle control gene of the fission yeast Schizosaccharomyces pombe.
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Site-specific mutagenesis of cdc2+, a cell cycle control gene of the fission yeast Schizosaccharomyces pombe.

机译：cdc2 +（裂变酵母粟酒裂殖酵母的细胞周期控制基因）的位点特异性诱变。

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The cdc2+ gene of Schizosaccharomyces pombe is homologous to the CDC28 gene of Saccharomyces cerevisiae. Both genes share limited homology with vertebrate protein kinases and have protein kinase activity. cdc2+ has been subjected to mutagenesis in vitro. A null allele of the gene, constructed by insertion of the S. cerevisiae LEU2 gene into a site within the gene, has a phenotype similar to that of many temperature-sensitive alleles of cdc2. Mutations within the predicted ATP-binding site and in a region which may be a site of phosphorylation result in loss of cdc2+ activity. A single substitution of Gly-146 to Asp-146 has been identified in cdc2-1w, a dominant activated allele of the gene. The four introns within the cdc2+ gene have been deleted. The resulting gene not only functions in fission yeast but also rescues cdc28(Ts) strains of S. cerevisiae, a property which is not shared by the genomic cdc2+ gene.
机译：粟酒裂殖酵母的cdc2 +基因与酿酒酵母的CDC28基因同源。这两个基因与脊椎动物蛋白激酶具有有限的同源性，并具有蛋白激酶活性。 cdc2 +已在体外进行诱变。该基因的无效等位基因是通过将酿酒酵母LEU2基因插入该基因内的一个位点而构建的，其表型与cdc2的许多温度敏感性等位基因相似。预测的ATP结合位点内和可能是磷酸化位点的区域内的突变会导致cdc2 +活性丧失。已经在cdc2-1w（该基因的显性激活等位基因）中鉴定了Gly-146单一替换为Asp-146。 cdc2 +基因中的四个内含子已被删除。产生的基因不仅在裂殖酵母中起作用，而且还拯救了酿酒酵母的cdc28（Ts）菌株，而基因组cdc2 +基因没有这种特性。

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