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首页> 外文期刊>Molecular and Cellular Biology >Permeabilization of ultraviolet-irradiated Chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus.
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Permeabilization of ultraviolet-irradiated Chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus.

机译：用聚乙二醇对紫外线照射的中国仓鼠细胞进行透化，并从黄球菌中引入紫外线内切核酸酶。

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摘要

Chinese hamster V-79 cells were made permeable by treatment with polyethylene glycol and then incubated with a Micrococcus luteus extract containing ultraviolet-specific endonuclease activity. This treatment introduced nicks in irradiated, but not in unirradiated, deoxyribonucleic acid. The nicks remained open for at least 3 h; there was no loss of endonuclease-sensitive sites, and no excision of dimers as measured by chromatography was detected. In addition, there was no increase in ultraviolet resistance in treated cells. This suggests that the absence of a significant amount of excision repair in rodent cells is due to the lack of both incision and excision capacity.
机译：通过用聚乙二醇处理使中国仓鼠V-79细胞具有可渗透性，然后与含有紫外线特异性核酸内切酶活性的黄褐微球菌提取物一起孵育。该处理在辐照的但不是未辐照的脱氧核糖核酸中引入了切口。缺口至少打开3小时;没有损失核酸内切酶敏感位点，也没有检测到通过色谱法测定的二聚体的切除。另外，在处理过的细胞中抗紫外线性没有增加。这表明在啮齿动物细胞中没有大量的切除修复是由于缺乏切口和切除能力。

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