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首页> 外文期刊>Molecular and Cellular Biology >Isolation of a cDNA clone complementary to sequences for a 34-kilodalton protein which is a pp60v-src substrate.
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Isolation of a cDNA clone complementary to sequences for a 34-kilodalton protein which is a pp60v-src substrate.

机译:cDNA克隆的互补序列,该克隆与pp60v-src底物34肽的序列互补。

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We have isolated a partial cDNA clone containing sequences complementary to a mRNA encoding a 34- to 36-kilodalton normal chicken cell protein which is a substrate for pp60v-src kinase activity. Using this 34-kilodalton cDNA clone as a probe, we determined that the size of the 34-kilodalton mRNA was 1,100 nucleotides and the level of the 34-kilodalton RNA was the same in various tissues of mature chickens but was significantly higher in chicken embryo fibroblast cells.
机译:我们已经分离出部分cDNA克隆,其中包含与编码34-36千达尔顿正常鸡细胞蛋白的mRNA互补的序列,该蛋白是pp60v-src激酶活性的底物。使用该34公斤的cDNA克隆作为探针,我们确定34公斤的mRNA的大小为1,100个核苷酸,而34公斤的RNA的水平在成熟鸡的各种组织中相同,但在鸡胚中显着更高成纤维细胞。

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