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首页> 外文期刊>Molecular and Cellular Biology >Promoter and enhancer elements from the rat elastase I gene function independently of each other and of heterologous enhancers.
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Promoter and enhancer elements from the rat elastase I gene function independently of each other and of heterologous enhancers.

机译：大鼠弹性蛋白酶I基因的启动子和增强子元件彼此之间以及异源增强子之间均独立起作用。

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摘要

An elastase-human growth hormone (hGH) fusion gene containing 205 base pairs of elastase 5' flanking region is expressed exclusively in pancreatic acinar cells of transgenic mice. This paper shows that the promoter region (-72 to +8) and the enhancer (-205 to -73) function independently of each other. The elastase enhancer can activate the heterologous mouse metallothionein gene and the hGH gene promoters; conversely, enhancers from the thymocyte-specific murine leukemia virus MCF13 and the metal regulatory elements from the metallothionein gene can activate the elastase promoter in a variety of cell types. Combinations of immunoglobulin and elastase enhancers with a heterologous promoter and the hGH gene result in expression in all of the tissues predicted by the sum of each enhancer acting alone. Thus these enhancer elements act independently of each other, suggesting that they do not have silencing activity in cells in which they are normally inactive.
机译：包含205个碱基对的弹性蛋白酶5'侧翼区的弹性蛋白酶-人类生长激素（hGH）融合基因仅在转基因小鼠的胰腺腺泡细胞中表达。本文显示启动子区域（-72至+8）和增强子（-205至-73）彼此独立起作用。弹性蛋白酶增强剂可以激活异源小鼠金属硫蛋白基因和hGH基因启动子。相反，来自胸腺细胞特异性鼠白血病病毒MCF13的增强子和来自金属硫蛋白基因的金属调节元件可以激活多种细胞类型中的弹性蛋白酶启动子。免疫球蛋白和弹性蛋白酶增强剂与异源启动子和hGH基因的组合导致在所有组织中的表达，这是由每个单独起作用的增强剂的总和预测的。因此，这些增强子元件彼此独立地起作用，表明它们在通常不活跃的细胞中不具有沉默活性。

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