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首页> 外文期刊>Molecular and Cellular Biology >Cell-specific expression of the human gastrin gene: evidence for a control element located downstream of the TATA box.
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Cell-specific expression of the human gastrin gene: evidence for a control element located downstream of the TATA box.

机译：人胃泌素基因的细胞特异性表达：位于TATA盒下游的控制元件的证据。

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摘要

Fragments of 5'-flanking and noncoding exon I sequences of the human gastrin gene were analyzed in transient expression assays after transfection of a variety of cell lines with the pSVCAT vector system. In the presence of the simian virus 40 (SV40) enhancer, the gastrin gene fragment from nucleotides -250 to +57, relative to the cap site, was as efficient a promoter as the SV40 early promoter itself. In the absence of the SV40 enhancer, gastrin gene 5'-flanking sequences had no promoter activity except in the murine neuroblastoma cell line N18TG2. In this cell line, the fragment from -1300 to +57 stimulated transcription as actively as the SV40 early promoter with its enhancer. This cell-specific gastrin gene promoter activity was in accordance with the finding that gastrin is synthesized in certain neuronal cells. Promoter activity declined with decreasing distance from the 5' end to the cap site and disappeared after removal of the gastrin gene TATA box. In vector constructions containing short vector-linker sequences homologous to a functionally important region of the SV40 enhancer, the gastrin gene fragment from -17 to +57 showed considerable promoter activity, exclusively in N18TG2. It is concluded that the truncated gastrin gene promoter plus the first exon contains a cell-specific element that may act in collaboration with upstream elements to facilitate the accumulation of transcripts.
机译：用pSVCAT载体系统转染多种细胞系后，在瞬时表达分析中分析了人胃泌素基因的5'侧翼和非编码外显子I序列的片段。在存在猿猴病毒40（SV40）增强子的情况下，相对于帽位点，核苷酸-250至+57的胃泌素基因片段与SV40早期启动子本身一样有效。在缺少SV40增强子的情况下，胃泌素基因5'侧翼序列除了在鼠神经母细胞瘤细胞系N18TG2中没有启动子活性。在该细胞系中，-1300至+57片段与具有其增强子的SV40早期启动子一样积极地刺激转录。这种细胞特异性胃泌素基因启动子活性与在某些神经元细胞中合成胃泌素的发现一致。启动子活性随着从5'末端到帽位点的距离的减小而下降，并且在去除胃泌素基因TATA框后消失。在含有与SV40增强子的功能重要区域同源的短载体-连接子序列的载体构建中，从-17至+57的胃泌素基因片段显示出相当大的启动子活性，仅在N18TG2中。结论是，截短的胃泌素基因启动子加上第一个外显子包含细胞特异性元件，该元件可以与上游元件协同作用以促进转录物的积累。

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