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首页> 外文期刊>Molecular and Cellular Biology >Saccharomyces cerevisiae PHO5 promoter region: location and function of the upstream activation site.
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Saccharomyces cerevisiae PHO5 promoter region: location and function of the upstream activation site.

机译：酿酒酵母PHO5启动子区域：上游激活位点的位置和功能。

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Saccharomyces cerevisiae repressible acid phosphatase (PHO5) is induced when inorganic phosphate in the culture medium is depleted. To study the mechanism of this regulation, we constructed various deletions in the 5'-flanking region of the PHO5 gene. Two elements were revealed by this analysis: an upstream activation site (UAS) and a downstream element, both playing parts in the expression of this gene. The UAS is located between -384 and -292 upstream of the initiation codon and activates expression of the gene when inorganic phosphate is depleted. It consists of two homologous regions (UAS I and UAS II) that contain CTGCACAAATG and an adenine-plus-thymine-rich sequence, either one of which suffices for the effect. The downstream element includes a putative TATA box at -100 from the ATG codon and is necessary for efficient transcription and expression of the normal-sized PHO5 transcript. The distance between the UAS and the downstream element can be altered without causing loss of expression efficiency, and the action of the UAS is not affected by its orientation. These results are consistent with a model wherein UAS acts as a site of activation for transcription by interaction with a protein factor(s) that becomes active when inorganic phosphate is depleted from the culture medium.
机译：当培养基中的无机磷酸盐耗尽时，会诱导酿酒酵母可抑制性酸性磷酸酶（PHO5）。为了研究这种调节的机制，我们在PHO5基因的5'侧翼区域构建了各种缺失。该分析揭示了两个要素：上游激活位点（UAS）和下游要素，两者均在该基因的表达中起作用。 UAS位于起始密码子上游的-384至-292之间，并在无机磷酸盐耗尽时激活基因的表达。它由两个同源区域（UAS I和UAS II）组成，其中包含CTGCACAAATG和富含腺嘌呤加胸腺嘧啶的序列，其中任何一个都足以满足上述要求。下游元件在ATG密码子的-100处包含一个假定的TATA盒，对于有效转录和表达正常大小的PHO5转录本是必需的。可以改变UAS与下游元件之间的距离而不会引起表达效率的损失，并且UAS的作用不受其方向的影响。这些结果与模型一致，在该模型中，UAS通过与蛋白质因子相互作用而充当转录激活位点，而当从培养基中消耗掉无机磷酸盐时，蛋白质因子就变得具有活性。

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