• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Functional domains of a negative regulatory protein, GAL80, of Saccharomyces cerevisiae.
【24h】

Functional domains of a negative regulatory protein, GAL80, of Saccharomyces cerevisiae.

机译：酿酒酵母阴性调节蛋白GAL80的功能域。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To study the functional domains of a transcriptional repressor encoded by the GAL80 gene of Saccharomyces cerevisiae, we constructed various deletion and insertion mutations in the GAL80 coding region and determined the ability of these mutations to repress synthesis of galactose-metabolizing enzymes as well as the capacity of the mutant proteins to respond to the inducer. Two regions, from amino acids 1 to 321 and from amino acids 341 to 423, in the total sequence of 435 amino acids were required for repression. The internal region from amino acids 321 to 340 played a role in the response to the inducer. The 12 amino acids at the carboxy terminus were dispensable for normal functioning of the GAL80 protein. Using indirect immunofluorescence and subcellular fractionation techniques, we also found that two distinct regions (amino acids 1 to 109 and 342 to 405) within the putative repression domain were capable of directing cytoplasmically synthesized Escherichia coli beta-galactosidase to the yeast nucleus. In addition, three gal80 mutations were mapped at amino acid residues 183, 298, and 310 in the domain required for repression. On the basis of these results, we suggest that the GAL80 protein consists of a repression domain located in two separate regions (amino acid residues 1 to 321 and 341 to 423) that are interrupted by an inducer interaction domain (residues 322 to 340) and two nuclear localization domains (1 to 109 and 342 to 405) that overlap the repression domains.
机译：为了研究由酿酒酵母的GAL80基因编码的转录阻遏物的功能域，我们在GAL80编码区构建了各种缺失和插入突变，并确定了这些突变抑制半乳糖代谢酶合成的能力以及能力。突变蛋白对诱导物的应答。抑制需要总共435个氨基酸中的两个区域，从1到321位氨基酸和341到423位氨基酸。氨基酸321至340的内部区域在对诱导物的应答中起作用。对于GAL80蛋白的正常功能而言，羧基末端的12个氨基酸是必需的。使用间接免疫荧光和亚细胞分级分离技术，我们还发现推定的抑制域内的两个不同区域（氨基酸1至109和342至405）能够将细胞质合成的大肠杆菌β-半乳糖苷酶导向酵母核。此外，三个gal80突变被定位在阻抑所需域中的氨基酸残基183、298和310处。根据这些结果，我们建议GAL80蛋白由位于两个独立区域（氨基酸残基1至321和341至423）中的阻遏域组成，该区域被诱导物相互作用域（残基322至340）打断，与抑制域重叠的两个核定位域（1至109和342至405）。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号