Repair of single-stranded DNA nicks, gaps, and loops in mammalian cells.

D Ayares; D Ganea; L Chekuri; C R Campbell; R Kucherlapati

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Repair of single-stranded DNA nicks, gaps, and loops in mammalian cells.

机译：修复哺乳动物细胞中单链DNA的缺口，缺口和环。

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We studied the ability of mammalian cells to repair single-stranded nicks, gaps, and loops in DNA duplexes. Heteroduplexes prepared from derivatives of the shuttle vector pSV2neo were introduced into monkey COS cells. After replication, the plasmids were recovered and used to transform Escherichia coli. Plasmid DNA from the recovered colonies was tested for repair at each of six different sites. We observed that mammalian cells are capable of repairing single-stranded gaps and free single-stranded ends most efficiently. Regions containing twin loops were recognized, and one of the loops was excised. Portions of the molecules containing small single loops were also repaired. Markers which were 58 nucleotides apart were corepaired with nearly 100% efficiency, while markers which were 1,000 nucleotides or more apart were never corepaired. The mechanisms involved in heteroduplex repair in mammalian cells seem to be similar to those involved in repairing DNA lesions caused by physical and chemical agents.

机译：我们研究了哺乳动物细胞修复DNA双链体中单链缺口，缺口和环的能力。由穿梭载体pSV2neo的衍生物制备的异源双链体被引入猴COS细胞。复制后，回收质粒并用于转化大肠杆菌。测试了来自回收菌落的质粒DNA在六个不同位点的修复。我们观察到哺乳动物细胞能够最有效地修复单链缺口和游离的单链末端。识别包含双环的区域，并切除其中一个环。含有小单环的分子部分也被修复。相距58个核苷酸的标记以几乎100％的效率进行核心配对，而相距1,000个核苷酸或更多的标记从未进行核心配对。哺乳动物细胞中异源双链体修复的机制似乎与物理和化学试剂引起的DNA损伤的修复机制相似。

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《Molecular and Cellular Biology》 |1987年第5期|共7页
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D Ayares; D Ganea; L Chekuri; C R Campbell; R Kucherlapati;
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中图分类细胞生物学;
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1. Repair of DNA strand gaps and nicks containing 3'-phosphate and 5'-hydroxyl termini by purified mammalian enzymes. [J] . Karimi-Busheri F, Lee J, Tomkinson AE, Nucleic Acids Research . 1998,第19期

机译：通过纯化的哺乳动物酶修复包含3'-磷酸和5'-羟基末端的DNA链缺口和缺口。
2. Repair of DNA strand gaps and nicks containing 3′-phosphate and 5′-hydroxyl termini by purified mammalian enzymes [J] . Alan E. Tomkinson, Feridoun Karimi-Busheri, Jane Lee, Nucleic acids research . 1998,第19期

机译：通过纯化的哺乳动物酶修复包含3'-磷酸和5'-羟基末端的DNA链缺口和缺口
3. Granzyme K cleaves the nucleosome assembly protein SET to induce single-stranded DNA nicks of target cells. [J] . Zhao T, Zhang H, Guo Y, Cell death and differentiation . 2007,第3期

机译：颗粒酶K裂解核小体装配蛋白SET，以诱导靶细胞的单链DNA缺口。
4. Targeted gene repair: from RNA/DNA to single-stranded oligonucleotides [C] . W. YIN, B. T. KREN, C. J. STEER Falk Symposium . 2004

机译：靶向基因修复：从RNA / DNA到单链寡核苷酸
5. The Rad51d DNA repair gene is required for chromosome and telomere stability in mammalian cells. [D] . Smiraldo, Phillip G. 2006

机译：Rad51d DNA修复基因是哺乳动物细胞中染色体和端粒稳定所必需的。
6. Repair of single-stranded DNA nicks gaps and loops in mammalian cells. [O] . D Ayares, D Ganea, L Chekuri, 1987

机译：修复哺乳动物细胞中单链DNA的缺口缺口和环。
7. Repair of single-stranded DNA nicks, gaps, and loops in mammalian cells. [O] . Ayares, D, Ganea, D, Chekuri, L, 1987

机译：修复哺乳动物细胞中单链DNA的缺口，缺口和环。
8. Molecular Targets, DNA Breakage, DNA Repair: Their Roles in Mutation Induction in Mammalian Germ Cells. [R] . Sega, G. A. 1989

机译：分子靶，DNa断裂，DNa修复：它们在哺乳动物生殖细胞突变诱导中的作用。

Repair of single-stranded DNA nicks, gaps, and loops in mammalian cells.

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