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首页> 外文期刊>Molecular and Cellular Biology >Genetic analysis of the human thymidine kinase gene promoter.
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Genetic analysis of the human thymidine kinase gene promoter.

机译：人类胸苷激酶基因启动子的遗传分析。

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The promoter of the human thymidine kinase gene was defined by DNA sequence and genetic analyses. Mutant plasmids with deletions extending into the promoter region from both the 5' and 3' directions were constructed. The mutants were tested in a gene transfer system for the ability to transform TK- cells to the TK+ phenotype. This analysis delimited the functional promoter to within an 83-base-pair region upstream of the mRNA cap site. This region contains sequences common to other eucaryotic promoters including G X C-rich hexanucleotides, a CAAT box, and an A X T-rich region. The CAAT box is in an inverted orientation and is part of a 9-base-pair sequence repeated twice in the promoter region. Comparison of the genomic sequence with the cDNA sequence defined the first exon of the thymidine kinase gene.
机译：人胸苷激酶基因的启动子通过DNA序列和遗传分析确定。构建具有从5'和3'方向延伸到启动子区域中的缺失的突变质粒。在基因转移系统中测试了突变体将TK细胞转化为TK +表型的能力。该分析将功能启动子限定在mRNA帽位点上游的83个碱基对区域内。该区域包含其他真核启动子共有的序列，包括富含G C的六核苷酸，CAAT框和富含A X T的区域。 CAAT盒处于反向方向，并且是在启动子区域中重复两次的9碱基对序列的一部分。基因组序列与cDNA序列的比较确定了胸苷激酶基因的第一个外显子。

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